EFFECT OF CURCUMA XANTHORRHIZA ROXB. ETHANOL EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS AND STREPTOCOCCUS SANGUINIS DUAL-SPECIES BIOFILMS
DOI:
https://doi.org/10.22159/ijap.2019.v11s1.181Keywords:
Dual species, Curuma xanthorriza ethanol extract, Streptococcus mutans, Streptococcus sanguinis, ViabilityAbstract
Objective: This study aimed to determine the effect of ethanol Curcuma extract on the viability of S. mutans and Streptococcus sanguinis in a dualspecies
in vitro biofilm model.
Methods: Dual-species biofilms of S. mutans and S. sanguinis were exposed to ethanol Curcuma extract at various concentrations. The sample of
saliva was gathered from healthy volunteers. Chlorhexidine 0.2% was used as a positive control, and bacterial culture without intervention served
as a negative control. The total suspensions of 10−as were prepared for S. mutans and S. sanguinis cells. The bacteria were incubated for 20 h (active
maturation phase) and 24 h (maturation phase).
Results: The result showed decreased S. mutans and S. sanguinis viability after exposure to 0.2%–25% Curcuma ethanol extracts during the active
accumulation and maturation phases. The decrease in bacterial viability was significantly different in all concentrations of Curcuma ethanol extracts
compared with negative controls (p<0.05) in the active accumulation and maturation phases.
Conclusion: Temulawak ethanol extract (starting at 0.2%) can decrease the viability of S. mutans and S. sanguinis in a dual species in vitro biofilm
model during the accumulation and maturation phases.
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