EFFECT OF BREADFRUIT LEAF EXTRACT ON THE VIABILITY OF STREPTOCOCCUS MUTANS BIOFILM IN VITRO

SEPTI WARDA ZULFIKAR; SRI UTAMI; RATNA FARIDA

doi:10.22159/ijap.2019.v11s1.AR201

Authors

SEPTI WARDA ZULFIKAR Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jalan Salemba Raya No.4, Jakarta Pusat, 10430, Indonesia.
SRI UTAMI Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jalan Salemba Raya No.4, Jakarta Pusat, 10430, Indonesia.
RATNA FARIDA Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jalan Salemba Raya No.4, Jakarta Pusat, 10430, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2019.v11s1.AR201

Keywords:

Extract of breadfruit leaf, Streptococcus mutans, Viability, MTT assay

Abstract

Objective: Breadfruit leaf has potent antibacterial properties that could be used to reduce biofilms in the oral cavity. The purpose of this study was to
analyze the antibacterial effect of the breadfruit leaf extract on the growth of Streptococcus mutans in vitro.
Methods: S. mutans ATCC 25175 was cultured in a 96-well plate and incubated at 37°C for 20 h (accumulation phase) and 24 h (maturation phase).
The breadfruit leaf extract was added at the following concentrations: 5%, 10%, 15%, 20%, 40%, 80%, and 100%. The viability of S. mutans was tested
with the MTT assay at a wavelength of 490 nm. The results were analyzed by one-way analysis of variance.
Results: In the accumulation phase, a significant decrease was found in S. mutans viability at different concentrations of the breadfruit leaf extract, but
in the maturation phase, a significant decrease was found in the S. mutans viability at the 5% concentration. The other groups decreased significantly
compared with the control group (*p<0.05). The viability of S. mutans after adding the breadfruit leaf extract at all concentrations was lower in the
accumulation phase than that in the maturation phase.
Conclusion: In the accumulation phase, breadfruit leaf extract at concentrations of 5%, 10%, 20%, 40%, 80%, and 100% can reduce S. mutans biofilm
viability.

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References

1. Katno, Pramono S. The Benefits and Safety of Medicinal Plants and
Traditional Medicines. Yogyakarta: Balai Penelitian Tanaman Obat
Tawangmangu, Fakultas Farmasi UGM; 2005. p. 8-9.
2. Mataharinews. Cancer Treatment with Breadfruit Leaves. Available
from: http://www.mataharinews.com/kesehatan/obat-penyakit/1743-
mengobati-kanker-dengan-daun-sukun.html.
3. Litbang Deptan Sukun. National Workshop on Breadfruit Development.
Available from: http://www.pustaka.litbang.deptan.go.id/publikasi/
wr11093.pdf.
4. Mount G, Hume WR. Dental Caries. Preservation and Restoration of
Tooth Structure. 2nd ed. Queensland: Knowledge Books and Software;
2005. p. 21-5.
5. Wen ZT, Yates D, Ahn SJ, Burne RA. Biofilm formation and virulence
expression by Streptococcus mutans are altered when grown in dualspecies
model. BMC Microbiol 2010;10:111.
6. Michalek SM, McGhee JR. Virulence of Streptococcus mutans: An
antibiotic-suppressed rat model for studies of pathogenesis. J Dent Res
1977;56:205-11.
7. Prescott LM, Harley JP, Klein DA. Microbiology. 5th ed. Boston:
McGraw-Hill; 2002. p. 602-21.
8. Abdassah M, Sumiwi SA, Hendrayana J. Breadfruit leaf extract
formulation with gel base as anti-inflammatory. J Farm Indones
2009;4:199-209.
9. Pankey GA, Sabath LD. Clinical relevance of bacteriostatic versus
bactericidal mechanisms of action in the treatment of gram-positive
bacterial infections. Clin Infect Dis 2004;38:864-70.
10. Purnamasari DA, Munadziroh E, Yogiartono RM. The concentration
of cocoa bean extract as a natural material in inhibiting the growth of
Streptococcus mutans. J PDGI 2010;59:1-18.
11. Kusdarwati R, Sari L, Mukti AT. Antibacterial power of fennel fruit
extract (Foeniculum vulgare) against Microccus luteus bacteria in vitro.
J Ilmiah 2010;2:35-31.
12. Suwandi T. Development of the Antibacterial Potential of Hibiscus
sabdariffa L. (Rosela) Towards Streptococcus Inducing Gingivitis
towards Standard Herbal Medicine [Dissertation]. Jakarta: Universitas
Indonesia; 2012.
13. Sabir A. Antibacterial activity of Trigona Sp towards Streptococcus
mutans (in vitro). Maj Ked Gigi 2005;38:135-41.
14. Mah TF, O’Toole GA. Mechanisms of biofilm resistance to antimicrobial
agents. Trends Microbiol 2001;9:34-9.
15. Jawettz E, Melnick JL, Adelberg EA. Medical Microbiology. 9th ed.
USA: Lange Medical Book; 1991. p. 149-52.
16. Hidayaningtias P. Comparison of the Antibacterial Effect of Steeping
Betel Leaf (Piper betle Linn) towards Streptococcus mutans at
Different Contact Times and Concentrations [Dissertation]. Semarang:
Universitas Diponegoro; 2008.
17. Rachmawati F, Nuria MC, Sumantri. Antibacterial Activity Test for
Chloroform Fraction of (Centella asiatica (L) Urb) Ethanol Extract and
Indentification of its Active Compounds. [Dissertation]. Yogyakarta:
Universitas Gadjah Mada; 2011.