DEVELOPMENT AND IN VITRO EVALUATION OF VALSARTAN-LOADED RESEALED ERYTHROCYTES

GAMAL OSMAN ELHASSAN; JAMAL MOIDEEN MUTHU MOHAMED

doi:10.22159/ijap.2022.v14ti.57

Authors

GAMAL OSMAN ELHASSAN Department of Pharmaceutics, Unaizah College of Pharmacy, Qassim University, Unaizah 51911, Saudi Arabia
JAMAL MOIDEEN MUTHU MOHAMED Vaasudhara College of Pharmacy, Sante Circle, Chintamani Road, Hoskote 562114, Karnataka, India

DOI:

https://doi.org/10.22159/ijap.2022.v14ti.57

Keywords:

Resealed erythrocytes, Valsartan, Cross-linking, Osmotic fragility

Abstract

Objective: Resealed erythrocyte technique has attempts for preparation of valsartan loaded reservoir type using red blood cell as a carrier.

Methods: The resealed erythrocytes were prepared by using glutaraldehyde as a cross-linking agent by Preswell dilution technique and sodium chloride as the medium has been made to predict mechanism of drug release and absorption based on the order of release. The prepared resealed erythrocytes was characterised as a percentage of cell recovery drug content, osmotic shock, turbulence fragility, osmotic fragility, in vitro drug release studies, and hemoglobin content study.

Results: The result of the study showed that the resealed erythrocytes prepared with sodium chloride (9 %) showed biconcave shape, an assay of 6.5±0.4 %, osmotic shock (0.028±0.004 µg/ml), turbulence fragility of 0.228±0.046 µg/ml, and 44.38±5.54 % drug release in 8 h. The drug release kinetics was studied and found that release from spherical matrices, first-order model with non-fickian diffusion with and the dissolution occurs in planes that are parallel to the drug surface pattern.

Conclusion: VaL administration could be avoided the number of drawbacks associated with systemic delivery and perhaps maintain a relatively constant plasma level during a lengthy course of treatment.

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