EFFECT OF COWANIN ON CYCLIN D1 EXPRESSION IN MCF-7/HER2 BREAST CANCER CELLS
DOI:
https://doi.org/10.22159/ijap.2024.v16s1.32Keywords:
Cowanin, Garcinia cowa Roxb, Cyclin D1, Cell cycle, Western blot, MCF-7/HER2 breast cancer cellsAbstract
Objective: Cowanin, isolated from the stem bark of Asam kandis (Garcinia cowa Roxb.) has been known to have cytotoxic activity in MCF-7/HER2 breast cancer cells. Recent studies have reported that cowanin compounds can inhibit MCF-7/HER2 cell migration and the T47D cell cycle in the G0-G1 phase. This research aims to determine the effect of cowanin on the cyclin D1 protein expression in MCF-7/HER2 breast cancer cells.
Methods: The treatment consisted of a negative control group and a group given cowanin at a concentration of IC50 value (10,51 µM). The expression of cyclin D1 protein was detected using the western blot method. Observations of protein area and density were carried out using ImageJ software. Data were analyzed using the independent T-test.
Results: The research showed that cowanin compounds induced cell cycle arrest of MCF-7/HER2 breast cancer cells by reducing the expression of cyclin D1 protein (p<0,05).
Conclusion: The findings show that cowanin can significantly decrease the area and density of cyclin D1 protein
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References
Sherr CJ. Cancer cell cycles. Science. 1996;274(5293):1672-7. doi: 10.1126/science.274.5293.1672, PMID 8939849.
Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71(3):209-49. doi: 10.3322/caac.21660, PMID 33538338.
Globocan. Cancer incident in Indonesia. International Agency for Research on Cancer; 2020.
Hanahan D. Hallmarks of cancer: new dimensions. Cancer Discov. 2022;12(1):31-46. doi: 10.1158/2159-8290.CD-21-1059, PMID 35022204.
Niu Y, Xu J, Sun T. Cyclin-dependent kinases 4/6 inhibitors in breast Cancer: current status, resistance, and combination strategies. J Cancer. 2019;10(22):5504-17. doi: 10.7150/jca.32628, PMID 31632494.
Alison MR. Cancer: encyclopedia of life sciences. Nature Publishing Group; 2001.
Gao X, Leone GW, Wang H. Cyclin D-CDK4/6 functions in cancer. Adv Cancer Res. 2020;148:147-69. doi: 10.1016/bs.acr.2020.02.002, PMID 32723562.
Siraj AK, Parvathareddy SK, Annaiyappanaidu P, Ahmed SO, Siraj N, Tulbah A. High expression of cyclin D1 is an independent marker for favorable prognosis in Middle Eastern breast cancer. Onco Targets Ther. 2021;14:3309-18. doi: 10.2147/OTT.S309091, PMID 34040395.
Freudenberg JA, Wang Q, Katsumata M, Drebin J, Nagatomo I, Greene MI. The role of HER2 in early breast cancer metastasis and the origins of resistance to HER2-targeted therapies. Exp Mol Pathol. 2009;87(1):1-11. doi: 10.1016/j.yexmp.2009.05.001, PMID 19450579.
Wahyuni FS, Shaari K, Stanslas J, Lajis NH, Hamidi D. Cytotoxic properties and complete nuclear magnetic resonance assignment of isolated xanthones from the root of garcinia cowa roxb. Pharmacogn Mag. 2016;12Suppl 1:S52-6. doi: 10.4103/0973-1296.176115, PMID 27041859.
Hefni D, Dachriyanus, Wahyuni FS, Yerizel E, Arisanty D, Yusra LN. Cowanin, a cytotoxic xanthone from asam kandis (Garcinia cowa, Roxb.) reduced cell migration and induced cell cycle arrest on T47D human cancer cell. International Journal on Advanced Science, Engineering and Information Technology 2020;10(5):2164-9. doi: 10.18517/ijaseit.10.5.12502.
Benz CC, Scott GK, Sarup JC, Johnson RM, Tripathy D, Coronado E. Estrogen-dependent, tamoxifen-resistant tumorigenic growth of MCF-7 cells transfected with HER2/neu. Breast Cancer Res Treat. 1992;24(2):85-95. doi: 10.1007/BF01961241, PMID 8095168.
Rezaei PF, Fouladdel S, Ghaffari SM, Amin G, Azizi E. Induction of G1 cell cycle arrest and cyclin D1 down-regulation in response to pericarp extract of Baneh in human breast cancer T47D cells. Daru. 2012;20(1):101. doi: 10.1186/2008-2231-20-101, PMID 23351343.
Collins JM, Wang D. Cytochrome P450 3A4 (CYP3A4) protein quantification using capillary Western blot technology and total protein normalization. J Pharmacol Toxicol Methods. 2021;112:107-17. doi: 10.1016/j.vascn.2021.107117, PMID 34474151.
Wahyuni FS, Shaari SJ, Lajis NH, Dachriyanus. Cytotoxic xanthones from the stem bark of garcinia cowa roxb. J Chem Pharm Res. 2015;7(1):227-36.
Vemu B, Nauman MC, Veenstra JP, Johnson JJ. Structure activity relationship of xanthones for inhibition of cyclin dependent kinase 4 from mangosteen (Garcinia mangostana L.). Int J Nutr. 2019;4(2):38-45. PMID 31363494.
Li G, Thomas S, Johnson JJ. Polyphenols from the mangosteen (Garcinia mangostana) fruit for breast and prostate cancer. Front Pharmacol. 2013;4(80):80. doi: 10.3389/fphar.2013.00080, PMID 23805102.
Zhu X, Li J, Ning H, Yuan Z, Zhong Y, Wu S. α-mangostin induces apoptosis and inhibits metastasis of breast cancer cells via regulating RXRα-AKT signaling pathway. Front Pharmacol. 2021;12:739658. doi: 10.3389/fphar.2021.739658, PMID 34539418.
Musgrove EA, Caldon CE, Barraclough J, Stone A, Sutherland RL. Cyclin D as A therapeutic target in cancer. Nat Rev Cancer. 2011;11(8):558-72. doi: 10.1038/nrc3090, PMID 21734724.
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