ANTIINFLAMMATORY OF MAIN COMPOUND FORM PIPER CROCATUM RUIZ AND PAV INHIBITION OF IL-1Β LIPOPOLYSACCHARIDE-INDUCED RAW 264,7 CELLS

NILDA LELY; FATMA SRI WAHYUNI; YUFRI ALDI; ALMAHDY

doi:10.22159/ijap.2022.v14s1.08

Authors

NILDA LELY Postgraduate Student, Faculty of Pharmacy, Andalas University, Padang, 25163, Indonesia, STIFI Bhakti Pertiwi, Palembang, 30128, Indonesia
FATMA SRI WAHYUNI Faculty of Pharmacy, Andalas University, Padang, 25163, Indonesia
YUFRI ALDI Faculty of Pharmacy, Andalas University, Padang, 25163, Indonesia
ALMAHDY Faculty of Pharmacy, Andalas University, Padang, 25163, Indonesia

DOI:

https://doi.org/10.22159/ijap.2022.v14s1.08

Keywords:

Antiinflamation, Piper crocatum, IL-1β, ELISA

Abstract

Objective: Inflammation is a physiological response to stimuli such as infection and tissue injury that causes an increase in the production of the proinflammatory cytokine IL-1β. Red betel has the potential as an anti-inflammatory drug, the aim study at to determine the anti-inflammatory effect of the main compound of red betel leaves.

Methods: Isolation of the main compound from the ethyl acetate fraction of red betel (Piper crocatum) leaves by column chromatography. Cell viability was determined by the MTT method in a concentration range of 1.25; 2.5; 5; 10; 20; 40 ug/ml. The anti-inflammatory effect of the main compound was tested against the inhibition of IL-1β production in LPS-induced RAW 264.7 macrophage cells. IL-1β levels were determined by ELISA.

Results: The viability test of the main compound from Piper crocatum leaves showed concentrations that gave viability percentages above 80%, namely 10; 5; 2.5; and 1.25 ug/ml. Concentrations of the main compound Piper crocatum leaves very significantly inhibited the production of IL-1β with p<0.001.

Conclusion: The main compound of Piper crocatum leaves has the potential to be used as an anti-inflammatory

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