CURCUMA AERUGINOSA ROXB. EXTRACT INHIBITS THE PRODUCTION OF PROINFLAMMATORY CYTOKINES ON RAW 264.7 MACROPHAGES

IRENE PUSPA DEWI; DACHRIYANUS; YUFRI ALDI; NOR HADIANI ISMAIL; DIRA HEFNI; MERI SUSANTI; SURYATI SYAFRI; FATMA SRI WAHYUNI

doi:10.22159/ijap.2024.v16s1.08

Authors

IRENE PUSPA DEWI Faculty of Pharmacy, Universitas Andalas, Indonesia -25163, Akademi Farmasi Prayoga, Indonesia, 25111 https://orcid.org/0000-0002-1857-9001
DACHRIYANUS Faculty of Pharmacy, Universitas Andalas, Indonesia -25163 https://orcid.org/0000-0002-1857-9001
YUFRI ALDI Faculty of Pharmacy, Universitas Andalas, Indonesia -25163
NOR HADIANI ISMAIL Atta-ur-Rahman Institute for Natural Product Discovery, UiTM Puncak Alam Campus, Malaysia https://orcid.org/0000-0002-1857-9001
DIRA HEFNI Faculty of Pharmacy, Universitas Andalas, Indonesia -25163
MERI SUSANTI Faculty of Pharmacy, Universitas Andalas, Indonesia -25163
SURYATI SYAFRI Faculty of Pharmacy, Universitas Andalas, Indonesia -25163 https://orcid.org/0000-0002-1857-9001
FATMA SRI WAHYUNI Faculty of Pharmacy, Universitas Andalas, Indonesia -25163 https://orcid.org/0000-0002-2026-4156

DOI:

https://doi.org/10.22159/ijap.2024.v16s1.08

Keywords:

Anti-inflammatory, Curcuma aeruginosa Roxb., Immune response, LPS, Raw 264.7 macrophages

Abstract

Objective: The study explores the potential of Curcuma aeruginosa Roxb. extract for anti-inflammatory properties.

Methods: Curcuma aeruginosa Roxb. simplicia was macerated with distilled ethanol. In vitro testing was done on Raw 264.7 macrophages to fulfill this aim by observing Tumor Necrosis Factor (TNF)-α, Interleukin (IL)-6 production and phagocytosis activity. The production of IL-6 and TNF-α were determined using the ELISA method while phagocytosis activity using the neutral red uptake method.

Results: The results showed that Curcuma aeruginosa Roxb. extract inhibited production of TNF-α and IL-6 and phagocytic activity and on Raw 264.7 macrophages.

Conclusion: The results demonstrated that Curcuma aeruginosa Roxb. extract could be developed as an anti-inflammatory, which can be improved as a novel pharmaceutical approach for treating inflammation-related illness.

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