ANALYSIS OF TURMERIC (CURCUMA LONGA LINN) ESSENTIAL OIL FROM DIFFERENT GROWING LOCATIONS USING FTIR/GC-MS SPECTROSCOPY COUPLED TO CHEMOMETRICS AND ITS WOUND HEALING ACTIVITIES

SURYATI SYAFRI; RISKANA SORAYA PUTRI; IRWANDI JASWIR; FARIDAH YUSOF; YOHANNES ALEN; SYOFYAN SYOFYAN; DACHRIYANUS HAMIDI

doi:10.22159/ijap.2024.v16s1.33

Authors

SURYATI SYAFRI Department of Pharmaceutical Biology, Faculty of Pharmacy. Universitas Andalas. Padang. West Sumatera, Indonesia
RISKANA SORAYA PUTRI Department of Pharmaceutical Biology, Faculty of Pharmacy. Universitas Andalas. Padang. West Sumatera, Indonesia
IRWANDI JASWIR International Institute of Halal Research and Training. International Islamic University Malaysia
FARIDAH YUSOF Departement of Biotechnology Engineering. Kulliyah of Engineering International Islamic University Malaysia
YOHANNES ALEN Department of Pharmaceutical Biology, Faculty of Pharmacy. Universitas Andalas. Padang. West Sumatera, Indonesia
SYOFYAN SYOFYAN Department of Pharmaceutical Technology, Faculty of Pharmacy. Universitas Andalas. Padang. West Sumatera, Indonesia
DACHRIYANUS HAMIDI Department of Pharmaceutical Biology, Faculty of Pharmacy. Universitas Andalas. Padang. West Sumatera, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024.v16s1.33

Keywords:

Turmeric, Essential oil, FTIR, GC-MS, Chemometrics, Wound healing

Abstract

Objective: This study aims to determine the wound-healing activity of turmeric essential oil (TEO) collected from seven growing locations in West Sumatra, classify it based on fingerprint patterns of IR spectra combined with chemometrics, and identify their metabolite profiling using GC-MS spectroscopy.

Methods: Fresh turmeric rhizome was extracted by the hydrodistillation method. TEO classification was carried out by PCA (Principal Component Analysis), and PLS-DA (Partial Least Squares-Discriminant Analysis) was used for predicting characteristic functional groups and metabolites (VIP>1) in TEO. Wound healing activity was performed using in vitro fibroblast cell proliferation and migration assay. Data analysis was performed using one-way ANOVA with a 95% confidence level.

Results: PCA analysis based FTIR spectra was able to determine highland and lowland-originated TEO. The metabolites responsible for TEO classification were α-Phellandrene and D-limonene. The result showed that TEO originating from both lowlands and highlands enhanced fibroblast cell proliferation and fibroblast cell migration.

Conclusion: The combination of IR spectral fingerprint patterns and chemometric analysis could classify TEO based on the height location of growth. The results showed that the altitude of the growing location had no significant effect on the wound-healing activity of TEO from West Sumatra (p>0.05).

Downloads

Download data is not yet available.

References

Cheppudira B, Fowler M, McGhee L, Greer A, Mares A, Petz L. Curcumin: a novel therapeutic for burn pain and wound healing. Expert Opin Investig Drugs. 2013;22(10):1295-303. doi: 10.1517/13543784.2013.825249, PMID 23902423.

Hamm RL. Drug-hypersensitivity syndrome: diagnosis and treatment. J Am Coll Clin Wound Spec. 2011;3(4):77-81. doi: 10.1016/j.jcws.2012.06.001, PMID 24527369.

Shetty V, Schwartz HC. Wound healing and perioperative care. Oral Maxillofac Surg Clin North Am. 2006;18(1):107-13. doi: 10.1016/j.coms.2005.09.004, PMID 18088815.

Addis R, Cruciani S, Santaniello S, Bellu E, Sarais G, Ventura C. Fibroblast proliferation and migration in wound healing by phytochemicals: evidence for a novel synergic outcome. Int J Med Sci. 2020;17(8):1030-42. doi: 10.7150/ijms.43986, PMID 32410832.

Syafri S, Husni E, Wafiqah N, Ramadhan F, Ramadani S, Hamidi D. Evaluation of antimicrobial and proliferation of fibroblast cells activities of citrus essential oils. Open Access Maced J Med Sci. 2022;10(A):1051-7. doi: 10.3889/oamjms.2022.8596.

Costa MF, Durço AO, Rabelo TK, de Barreto RSS, Guimarães AG. Effects of carvacrol, thymol and essential oils containing such monoterpenes on wound healing: a systematic review. J Pharm Pharmacol. 2019;71(2):141-55. doi: 10.1111/jphp.13054, PMID 30537169.

Komakech R, Matsabisa MG, Kang Y. The wound healing potential of Aspilia africana (Pers.) C.D. adams (Asteraceae). Evidence-Based Complementary and Alternative Medicine. 2019;2019:1-12. doi: 10.1155/2019/7957860.

Barra A. Factors affecting chemical variability of essential oils: a review of recent developments. Nat Prod Commun. 2009;4(8):1147-54. doi: 10.1177/1934578X0900400827, PMID 19769002.

Diliarosta S, Prima Sari M, Ramadhani R, Efendi A. We are Intech open, the world’s leading publisher of open-access books built by scientists for scientists TOP 1 %. Intech. 2016;13.

Arbain D. Inventory, constituents and conservation of biologically important Sumatran plants. Nat Prod Commun. 2012;7(6):799-806. doi: 10.1177/1934578X1200700627, PMID 22816311.

Ziaee M, Khorrami A, Ebrahimi M, Nourafcan H, Amiraslanzadeh M, Rameshrad M. Cardioprotective effects of essential oil of Lavandula angustifolia on isoproterenol-induced acute myocardial infarction in rat. Iran J Pharm Res. 2015;14(1):279-89. PMID 25561934.

Fahmi Z, Mudasir M, Rohman A. Attenuated total reflectance-FTIR spectra combined with multivariate calibration and discrimination analysis for analysis of patchouli oil adulteration. Indones J Chem. 2020;20(1):1-8. doi: 10.22146/ijc.36955.

Rohman A, Sudjadi, Devi RD, Ramadhani D, Nugroho A. Analysis of curcumin in Curcuma longa and Curcuma xanthorriza using FTIR spectroscopy and chemometrics. Res J Med Plants. 2015;9(4):179-86. doi: 10.3923/rjmp.2015.179.186.

Rohaeti E, Rafi M, Syafitri UD, Heryanto R. Fourier transform infrared spectroscopy combined with chemometrics for discrimination of Curcuma longa, curcuma xanthorrhiza and Zingiber cassumunar. Spectrochim Acta A Mol Biomol Spectrosc. 2015;137:1244-9. doi: 10.1016/j.saa.2014.08.139, PMID 25305617.

Widawati M, Nurjana MA, Mayasari R. Perbedaan dataran tinggi dan dataran rendah terhadap keberagaman spesies anopheles spp. di provinsi nusa tenggara timur. Aspirator J Vector Borne Dis Stud. 2018;10(2):103-10. doi: 10.22435/asp.v10i2.206.

Maree J, Kamatou G, Gibbons S, Viljoen A, Van Vuuren S. The application of GC-MS combined with chemometrics for the identification of antimicrobial compounds from selected commercial essential oils. Chemom Intell Lab Syst. 2014;130:172-81. doi: 10.1016/j.chemolab.2013.11.004.

Windarsih A, Nisa K, Indrianingsih AW, Darsih C, Handayani S, Wulanjati MP. The use of 1H-NMR spectroscopy and chemometrics of pattern recognition for authentication of Curcuma xanthorrhiza adulterated with Zingiber montanum. IOP Conf Ser.: Mater Sci Eng. 2021;1011(1):1-7. doi: 10.1088/1757-899X/1011/1/012050.

Jugreet BS, Suroowan S, Rengasamy RRK, Mahomoodally MF. Chemistry, bioactivities, mode of action and industrial applications of essential oils. Trends Food Sci Technol. 2020;101:89-105. doi: 10.1016/j.tifs.2020.04.025.

Figueiredo AC, Barroso JG, Pedro LG, Scheffer JJC. Factors affecting secondary metabolite production in plants: volatile components and essential oils. Flavour & Fragrance J. 2008;23(4):213-26. doi: 10.1002/ffj.1875.

Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I. Determination of the wound healing effect of calendula extracts using the scratch assay with 3T3 fibroblasts. J Ethnopharmacol. 2009;126(3):463-7. doi: 10.1016/j.jep.2009.09.014, PMID 19781615.

Juneja K, Mishra R, Chauhan S, Gupta S, Roy P, Sircar D. Metabolite profiling and wound-healing activity of Boerhavia diffusa leaf extracts using in vitro and in vivo models. J Tradit Complement Med. 2020;10(1):52-9. doi: 10.1016/j.jtcme.2019.02.002, PMID 31956558.

Freire G, Caldas R, Rodrigo A, Araujo AV, Sette S, Lafayette L. Gastroprotective mechanisms of the monoterpene 1, 8-cineole (eucalyptol); 2015.