PHYTOCHEMICAL ANALYSIS AND ANTIOXIDANT ACTIVITY OF METHANOL EXTRACT OF ZANTHOXYLUM ACANTHOPODIUM DC. FRUITS USING CUPRAC METHODS

AULIA ASSYFA; AMINAH DALIMUNTHE; MAHATIR MUHAMMAD; CHOO MEUN YOO; DENNY SATRIA

doi:10.22159/ijap.2024v16s4.52256

Authors

AULIA ASSYFA Department of Pharmaceutical Biology, Faculty of Pharmacy, Medan, Universitas Sumatera Utara, Indonesia https://orcid.org/0009-0001-7046-2759
AMINAH DALIMUNTHE Department of Pharmacology, Faculty of Pharmacy, Medan, Universitas Sumatera Utara, Indonesia
MAHATIR MUHAMMAD Faculty of Vocational, Medan, Universitas Sumatera Utara, Indonesia
CHOO MEUN YOO Department of Chemistry, Faculty of Science, Kuala Lumpur, Universiti Malaya, Malaysia
DENNY SATRIA Department of Pharmaceutical Biology, Faculty of Pharmacy, Medan, Universitas Sumatera Utara, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16s4.52256

Keywords:

Zanthoxylum acanthopodium DC, Antioxidant, Methanol extract, Phytochemical analysis, Hypertension

Abstract

Objective: Free radicals are substances that can be harmful to our bodies if their concentrations are too high. Free radicals are neutralized by antioxidant molecules, which additionally protect cells against damage from oxidation caused by free radicals. This study aims to conduct a phytochemical analysis and quantify the antioxidant capabilities of the methanol extract of Zanthoxylum acanthopodium DC. fruits.

Methods: Zanthoxylum acanthopodium DC. fruit was extracted by reflux method using methanol. Antioxidant activity for extract was measured with CUPRAC (Cupric Ion Reducing Antioxidant Capacity) method and LC-HRMS (Liquid Chromatography High Resolution Mass Spectrometry) instrument was used for phytochemical analysis of methanol extract.

Results: The of methanol extract was 86.58±1.87 μg/ml. LC-HRMS analysis showed that there were 10 active compounds detected in methanol extract of Zanthoxylum acanthopodium DC. The content of secondary metabolite compound was group of flavonoid (“neohesperidin, isorhamnetin, astragalin, quercetin, quercetin-3β-D-glucoside, (2E)-4-Hydroxy-3,7-dimethyl-2,6-octadien-1-yl beta-D-glucopyranoside, and 5,7-Dihydroxy-2-(4-hydroxyphenyl)-6,8-bis[3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl]-4H-chromen-4-one”) and phenolic (Chlorogenic acid, quinic acid).

Conclusion: The results show that the methanol extract from the fruit of Zanthoxylum acanthopodium DC. has potent antioxidant properties and contains several flavonoid and phenolic components.

Objective: Free radicals are substances that can be harmful to our bodies if their concentrations are too high. Free radicals are neutralized by antioxidant molecules, which additionally protect cells against damage from oxidation caused by free radicals. This study aims to conduct a phytochemical analysis and quantify the antioxidant capabilities of the methanol extract of Zanthoxylum acanthopodium DC. fruits.

Methods: Zanthoxylum acanthopodium DC. fruit was extracted by reflux method using methanol. Antioxidant activity for extract was measured with CUPRAC (Cupric Ion Reducing Antioxidant Capacity) method and LC-HRMS (Liquid Chromatography High Resolution Mass Spectrometry) instrument was used for phytochemical analysis of methanol extract.

Results: The of methanol extract was 86.58±1.87 μg/ml. LC-HRMS analysis showed that there were 10 active compounds detected in methanol extract of Zanthoxylum acanthopodium DC. The content of secondary metabolite compound was group of flavonoid (“neohesperidin, isorhamnetin, astragalin, quercetin, quercetin-3β-D-glucoside, (2E)-4-Hydroxy-3,7-dimethyl-2,6-octadien-1-yl beta-D-glucopyranoside, and 5,7-Dihydroxy-2-(4-hydroxyphenyl)-6,8-bis[3,4,5-trihydroxy-6-(hydroxymethyl) tetrahydro-2H-pyran-2-yl]-4H-chromen-4-one”) and phenolic (Chlorogenic acid, quinic acid).

Conclusion: The results show that the methanol extract from the fruit of Zanthoxylum acanthopodium DC. has potent antioxidant properties and contains several flavonoid and phenolic components.

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References

Jamuna S, Pulsamy S, Karthika K. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of Hypochaeris radicata L. (Asteraceae). J Appl Pharm Sci. 2012;2(7):149-54. doi: 10.7324/JAPS.2012.2722.

Nagmoti D, M Khatri, DK, Juvekar PR. Antioxidant activity free radical-scavenging potential of Pithecellobium dulce Benth seed extracts. Free Radic Antioxid. 2012;112(2):37-43. doi: 10.5530/ax.2012.2.2.7.

Rosidah MF, Yam A, Sadikun MZ, Asmawi. Antioxidant Potential of Gynura procumbens. Pharm Biol. 2008;46(3):616-25. doi: 10.1080/13880200802179642.

Yang YC, Lu FH, Wu JS, Wu CH. The protective effect of habitual tea consumption on hypertension. Arch Intern Med. 2004;164(2):1534. doi:10.1001/archinte.164.14.1534.

Suryanto E, Sastrohamidjojo H, Raharjo S, Tranggongo. Antiradical activity of andaliman (Zanthoxylum acanthopodium DC.) fruit extract. Indonesian Food Nutr Prog. 2004;11(1):15-9.

Hynniewta SR, Kumar Y. “Herbal remedies among the khasi traditional healers and village folk in Meghalaya. Indian J Trad Knowl. 2008;7(4):581.

Sirait M, Mangkudidjojo S. Post graduate school of pharmacy, institute technology of Bandung, Indonesian; 1991.

Hynniewta SR, Kumar Y. Herbal remedies among the Khasi traditional healers and village folks in Meghalaya. Indian J Trad Knowl. 2008;7(4):581-6.

Parhusip A, Ekstrak Andaliman KMA (Zanthoxylum acanthopodium DC.) terhadap Bakteri Patogen Pangan [tesis]. Institut Pertanian Bogor; 2006.

Fernandes CC, Vieira PC, da Silva VC, Dall’Oglio EL, da Silva LE, de Sousa PT Jr. 6-acetonyl-N-methyl-dihydrodecarine, a new alkaloid from Zanthoxylum riedelianum. J Braz Chem Soc. 2009;20(2):379-82. doi: 10.1590/S0103-50532009000200025.

Akoua Yao Kouassi P, Caron C, Ramiarantsoa H, Prost E, Harakat D, Le Magrex-Debar E. New nitro-benzo[c]phenanthridine and indolopyridoquinazoline alkaloids from Zanthoxylum atchoum. C R Chim. 2015;18(8):891-7. doi: 10.1016/j.crci.2015.01.005.

Hu J, Zhang WD, Liu RH, Zhang C, Shen YH, Li HL. Benzophenanthridine alkaloids from Zanthoxylum nitidum (Roxb.) DC, and their analgesic and anti-inflammatory activities. Chem Biodivers. 2006;3(9):990-5. doi: 10.1002/cbdv.200690108, PMID 17193331.

Kunwar A, Priyadarsini KI. Free radicals, oxidative stress, and importance of antioxidants in human health. J Med Allied Sci. 2011;1(2):53-60.

Satria D, Silalahi J, Haro G. Cell cycle inhibition of ethylacetate fraction of Zanthoxylum acanthopodium DC. fruit against T47d cells. Open acces mace. J Med Sci. 2019;7(5):726-9.

Satria D, Silalahi J, Haro G, Ilyas S, Zaitun Hasibuan PA. Chemical analysis and cytotoxic activity of nhexane fraction of Zanthoxylum acanthopodium DC. Fruits. Rasayan J Chem. 2019;12(2):803-8. doi: 10.31788/RJC.2019.1225180.

Amorati R, Valgimigli L. Methods to measure the antioxidant activity of phytochemicals and plant extracts. J Agric Food Chem. 2018;66(13):3324-9. doi: 10.1021/acs.jafc.8b01079, PMID 29557653.

Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. J Sci Technol. 2004;26(2):11-9.

Apak R, Guclu K, Demirata B, Ozyurek M, Celik SE, Bektaşoglu B. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules. 2007;12(7):1496-547. doi: 10.3390/12071496, PMID 17909504.

Toga Nugraha AT. Profil senyawa dan aktiftas antioksidan daun yakon (Smallanthus sonchifolius) dengan metode DPPH dan Cuprac. J Ilmiah Farmasi. 2017;13(1):15-8. doi: 10.20885/jif.vol13.iss1.art3.

Dontha S. A review of antioxidant methods. Asian J Pharm Clin Res. 2016;9(1):14-32.

Debanjali S, Sharma P, Baruah HS, Nath S, Akhtar S. Phytochemical screening and antioxidant activity of leaf extract of phlogacanthus thyrsiflorus nees,-a medicinal plant of Assam, India. Biosci Discov. 2018;237(9):43.

Hoang L, Benes F, Fenclova M, Kronusova O, Svarcova V, Rehorova K. Phytochemical composition and in vitro biological activity of Iris spp. (Iridaceae): a new source of bioactive constituents for the inhibition of oral bacterial biofilms. Antibiotics (Basel). 2020;9(7):403. doi: 10.3390/antibiotics9070403, PMID 32664528.

Maryam S, Pratama R, Effendi N, Naid T, Ekstrak Etanolik AAA, Yodium D. (Jatropha multifida L.) dengan metode cupric ion reducing antioxidant capcity (CUPRAC). J Fitofarmaka Indones. 2015;2(1):92-3.

Sadeer BN, Montesano D, Albrizio S, Zengin G, Mahomoodally FM. The versatility of antioxidant assays in food science and safety-chemistry, applications, strengths, and limitations. MDPI; 2020.

Hasibuan PA, Ilyas SR, Nasution MP. Antioxidant and cytotoxic activities of Plectranthus amboinicus (Lour.) spreng. extracts. Int J Pharm Teach Pract. 2013;755(4):8.

Zhang J, Wu Y, Zhao X, Luo F, Li X, Zhu H. Chemopreventive effect of flavonoids from Ougan (Citrus reticulata cv. Suavissima) fruit against cancer cell proliferation and migration. J Funct Foods. 2014;10(2):511-9. doi: 10.1016/j.jff.2014.08.006.

Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem. 2002;13(10):572-84. doi: 10.1016/S0955-2863(02)00208-5.

Kukic J, Petrovic S, Niketic M. Antioxidant activity of four endemic Stachys taxa. Biol Pharm Bull. 2006;29(4):725-9. doi: 10.1248/bpb.29.725, PMID 16595907.

Nijveldt RJ, van Nood E, van Hoorn DE, Boelens PG, van Norren K, van Leeuwen PA. Flavonoids: a review of probable mechanisms of action and potential applications. Am J Clin Nutr. 2001;74(4):418-25. doi: 10.1093/ajcn/74.4.418, PMID 11566638.

Karak P. Biological activities of flavonoids. Int J Pharm Sci Res. 2018;10(4):1570.

Lopez Lazaro ML. Distribution and biological activities of the flavonoid luteolin. Mini Rev Med Chem. 2009;9(1):31-59. doi: 10.2174/138955709787001712, PMID 19149659.

Murlidhar A, Babu KS, Sankar TR, Redenna P, Reddy GV, Latha J. Anti-inflammatory activity of flavonoid fraction isolated from stem bark of Butea monosperma (Lam.): a mechanism based study. Int J Phytopharmacol. 2010;1(114):124-32.

Pandey AK, Mishra AK, Mishra A. Antifungal and antioxidative potential of oil and extracts derived from leaves of Indian spice plant Cinnamomum tamala. Cell Mol Biol (Noisy-le-grand). 2012;58(1):142-7. PMID 23273204.

Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships. J Nutr Biochem. 2002;13(10):572-84. doi: 10.1016/s0955-2863(02)00208-5, PMID 12550068.