FORMULATION AND EVALUATION OF HERBAL CAPSULE CONTAINING CURCUMA MANGGA VALETON and ZIJP. EXTRACT AND ITS IMMUNOSTIMULATORY EFFECTS

Authors

  • YUANDANI Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia. Centre of Excellence for Chitosan and Advanced Materials, Universitas Sumatera Utara, Indonesia
  • LIA LAILA Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia. 2Centre of Excellence for Chitosan and Advanced Materials, Universitas Sumatera Utara, Indonesia https://orcid.org/0000-0002-9343-5518
  • SONY EKA NUGRAHA Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia https://orcid.org/0000-0001-8849-2481
  • WAHYU ARNIDA HASIBUAN Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia
  • RIFDA YUSRINA NASUTION Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia
  • DENNY SATRIA Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023v15i6.49061

Keywords:

Curcuma mangga, Formulation, Capsule, Immunomodulator

Abstract

Objective: The present study was carried out to formulate and evaluate C. mangga extract as granule mass in a capsule dosage form as well as investigate its immunostimulatory effects.

Methods: The granule mass was evaluated for its bulk density, tapped density, Carr’s index, Hausner ratio, angle of repose, and flow ability. Meanwhile, capsule weight and disintegration time were determined to evaluate the C. mangga capsule. The immunostimulatory effects of extract in capsule dosage form were investigated by measuring delayed-type hypersensitivity (DTH) response and IgG production. There were six formula prepared (F1-F6). Formula F1-F6 were vary according to the component of adsorbent (Corn starch/polyvinyl pyrrolidone) and filler (maltodextrin/Avicel/lactose) to obtain the optimum formula.

Results: The granule mass of all formula had a good flow ability and free-flowing properties according to the Carr’s index (10.26±3.38 to 17.54±1.60) and flowing time (1.05±0.05 to 1.60±0.08 seconds). Of all the capsule formula, F2 capsule containing dry extract of C. mangga with Avicel as the filler met the standard requirement in terms of disintegration time (2.15±0.76 min) and weight uniformity (the deviation was below than 7.5%). The capsule of C. mangga extract enhanced the paw thickness as compared to those of the negative control (P<0.05). The level of Immunoglobulin G (IgG) was also increased after treatment with the capsule of C. mangga extract.

Conclusion: The results suggest that the capsule of C. mangga extract has immunostimulatory effects, emphasizing its potential to be used for the treatment of infectious and immunodeficiency diseases.

Downloads

Download data is not yet available.

References

Levinson W. Review of medical microbiology and immunology. McGraw-Hill Education; 2016.

Saroj P, Verma M, Jha KK, Pal M. An overview on immunomodulation. J Adv Sci Res. 2012;3(1):7-12.

Luckheeram RV, Zhou R, Verma AD, Xia B. CD4⁺T cells: differentiation and functions. Clin Dev Immunol. 2012. doi: 10.1155/2012/925135, PMID 22474485.

Elgert KD. Immunology understanding the immune system. 2nd ed. Chichester: John Wiley & Sons; 2009. p. 305-95.

Tobon GJ, Izquierdo JH, Canas CA. B lymphocytes: development, tolerance, and their role in autoimmunity-focus on systemic lupus erythematosus. Autoimmune Dis. 2013;2013:827254. doi: 10.1155/2013/827254, PMID 24187614.

Schroeder HW, Cavacini L. Structure and function of immunoglobulins. J Allergy Clin Immunol. 2010;125(2)Suppl 2:S41-52. doi: 10.1016/j.jaci.2009.09.046, PMID 20176268.

Rahman MM, Mosaddik A, Alam AK. Traditional foods with their constituent’s antiviral and immune system modulating properties. Heliyon. 2021;7(1):e05957. doi: 10.1016/j.heliyon.2021.e05957, PMID 33462562.

Patil US, Jaydeokar AV, Bandawane DD. Immunomodulators: a pharmacological review. Int J Pharm Pharm Sci. 2012;4Suppl 1:30-6.

Abas F, Lajis NH, Shaari K, Israf DA, Stanslas J, Yusuf UK. A labdane diterpene glucoside from the rhizomes of Curcuma mangga. J Nat Prod. 2005;68(7):1090-3. doi: 10.1021/np0500171, PMID 16038556.

Yuandani YS, Satria D, Dongoran RF, Sinaga MS, Marpaung NHA, HA Marpaung N. Correlation between the phytochemical constituents of Curcuma mangga and its immunomodulatory effect. Rasayan J Chem. 2019;12(1):1-6. doi: 10.31788/RJC.2019.1215050.

Yuandani Yd, Suwarso E. Immunomodulatory effects of ethanol extract of curcuma mangga rhizomes in mice. Asian J Pharm Clin Res. 2017b;10(9):148-50. doi: 10.22159/ajpcr.2017.v10i9.18398.

Yuandani SE. Acute toxicity evaluation of ethanol extract of Curcuma mangga rhizome. Asian J Pharm Clin Res. 2017;10(1):383-5. doi: 10.22159/ajpcr.2017.v10i1.16196.

Abas F, Lajis NH, Israf DA, Khozirah S, Umi Kalsom YU. Antioxidant and nitric oxide inhibition activities of selected Malay traditional vegetables. Food Chem. 2006;95(4):566-73. doi: 10.1016/j.foodchem.2005.01.034.

Gibson M. Pharmaceutical preformulation and formulation. New York: Informa Healthcare; 2021.

Antonio A, Junior S, Carbinatto FM, Castro O. Preformulation studies of gymnema sylvestre extract powder formulation for hard gelatin capsules. Rev Ciênc Farm Básica Apl. 2011;32(2):175-80.

Indonesian pharmacopoeia. 3rd ed. Jakarta: Health Department of Republic of Indonesia; 1979.

Ahirwal L, Singh S, Dubey MK, Bharti V, Mehta A, Shukla S. In vivo immunomodulatory effects of the methanolic leaf extract of Gymnema sylvestre in Swiss albino mice. Arch Biol Sci (Beogr). 2015;67(2):561-70. doi: 10.2298/ABS141027018A.

Harahap U, Marianne M, Yuandani Y, Laila L. Preformulation study of Pugun Tano (Curanga fel-terrae [lour.] merr) ethanolic extract granule mass in the capsule as a hepatoprotective drug. Open Access Maced J Med Sci. 2019;7(22):3729-32. doi: 10.3889/oamjms.2019.491, PMID 32127963.

Aulton ME, Taylor KMG. Aulton’s pharmaceutics, book: the design and manufacture of medicines. Elsevier; 2018.

Mohammed SS. Preparation, characterization and preformulation studies of diltiazem sustain release tablet. Asian J Res Pharm Sci Biotechnol. 2014;2(1):16-22.

Published

07-11-2023

How to Cite

YUANDANI, LAILA, L., NUGRAHA, S. E., HASIBUAN, W. A., NASUTION, R. Y., & SATRIA, D. (2023). FORMULATION AND EVALUATION OF HERBAL CAPSULE CONTAINING CURCUMA MANGGA VALETON and ZIJP. EXTRACT AND ITS IMMUNOSTIMULATORY EFFECTS. International Journal of Applied Pharmaceutics, 15(6), 172–177. https://doi.org/10.22159/ijap.2023v15i6.49061

Issue

Section

Original Article(s)

Most read articles by the same author(s)