EFFECT OF POLYMER-INDUCED LIQUID PRECURSOR PROCESS CONTAINING POLYASPARTIC ACID ON INTRAFIBRILLAR DENTIN REMINERALIZATION (MICRO-COMPUTED TOMOGRAPHY ANALYSIS)
DOI:
https://doi.org/10.22159/ijap.2020.v12s2.OP-45Keywords:
Polyaspartic acid, Polymer-induced liquid precursor process, Non-collagen protein analog, Dentin remineralizationAbstract
Objective: Caries treatment can be performed by minimal intervention, i.e., by removing the infected dentin and leaving the affected dentin and then
inducing remineralization in the affected dentin. The affected dentin still consists of collagen cross bonds. Polymer-induced liquid precursor process
is a guided tissue remineralization method that aims to remineralize intrafibrillar and extrafibrillar dentin by adding polymers that are similar to
non-collagen protein. One of the non-collagen protein analog materials is polyaspartic acid. The aim of this study was to evaluate the remineralization
of dentin on the demineralized dentin surface after immersed in remineralization solutions containing polyaspartic acid as a non-collagen protein
analog.
Methods: Human premolar teeth extracted for orthodontic purposes were divided into four groups, namely, one control and three treatment groups.
Teeth in the control group were immersed only in the demineralization solution containing acetate buffer (pH 5.0, 66 h). Teeth in the three treatment
groups were immersed in acetate buffer (pH 5.0, 66 h) and then continue to immersed in the remineralization solution containing polyaspartic acid
for 3, 7, and 14 days. Remineralization was evaluated by micro-CT.
Results: Remineralization appeared on the demineralized dentin surface, characterized by an increase in the grayscale index, after immersion in
the remineralization solution containing polyaspartic acid. Significant differences were observed in the mean grayscale index values among the four
groups.
Conclusion: Polyaspartic acid has the potential to induce dentin remineralization.
Downloads
References
Pratiwi AR, Meidyawati R, Djauharie N. The effect of MTA application
on the affected dentine remineralization after partial caries excavation
(in vivo). J Phys Conf Ser 2017;884:012119.
Ricketts D, Lamont T, Innes NP, Kidd E, Clarkson JE. Operative caries
management in adults and children. Cochrane Database Syst Rev
;3:CD003808.
Vaseenon S. Relationship Between Caries-affected Dentin Mineral
Density and Microtensile Bond Strength. Iowa: University of Iowa;
Zhong B, Peng C, Wang G, Tian L, Cai Q, Cui F. Contemporary
research findings on dentine remineralization. J Tissue Eng Regen Med
;9:1004-16.
Gower LB. Biomimetic model systems for investigating the amorphous
precursor pathway and its role in biomineralization. Chem Rev
;108:4551-627.
Dai L, Liu Y, Salameh Z, Khan S, Mao J, Pashley DH, et al. Can
caries-affected dentin be completely remineralized by guided tissue
remineralization? Dent Hypotheses 2011;2:74-82.
Chen Z, Cao S, Wang H, Li Y, Kishen A, Deng X, et al. Biomimetic
remineralization of demineralized dentine using scaffold of CMC/ACP
nanocomplexes in an in vitro tooth model of deep caries. PLoS One
;10:e0116553.
Burwell AK, Thula-Mata T, Gower LB, Habelitz S, Kurylo M, Ho SP,
et al. Functional remineralization of dentin lesions using polymerinduced
liquid-precursor process. PLoS One 2012;7:e38852.
Fan Y, Sun Z, Moradian-Oldak J. Controlled remineralization of enamel in
the presence of amelogenin and fluoride. Biomaterials 2009;30:478-83.
Nudelman F, Pieterse K, George A, Bomans PH, Friedrich H,
Brylka LJ, et al. The role of coll in bone apatite form in the presence of
hydroxyapatite nucleation inhibitors. Nat Mater 2010;9:1004-9.
Krogstad DV, Wang D, Lin-Gibson S. Polyaspartic Acid Concentration
Controls the Rate of Calcium Phosphate Nanorod Formation in High
Concentration Systems. Biomacromolecules 2017;18(10):3106-13.
Nurrohman H, Carneiro KM, Hellgeth J, Saeki K, Marshall SJ,
Marshall GW, et al. The role of protease inhibitors on the
remineralization of demineralized dentin using the PILP method. PLoS
One 2017;12:e0188277.
Vollenweider M, Brunner TJ, Knecht S, Grass RN, Zehnder M,
Imfeld T, et al. Remineralization of human dentin using ultrafine
bioactive glass particles. Acta Biomater 2007;3:936-43.
Yu OY, Mei ML, Zhao IS, Lo EC, Chu CH. Effects of fluoride on
two chemical models of enamel demineralization. Materials (Basel)
;10:E1245.
Sullivan RJ, Charig A, Blake-Haskins J, Zhang YP, Miller SM,
Strannick M, et al. In vivo detection of calcium from dicalcium
phosphate dihydrate dentifrices in demtneralized human enamel and
plaque. Adv Dent Res 1997;11:380-7.
Goldberg M, Kulkarni AB, Young M, Boskey A. Dentin: Structure,
composition and mineralization. Front Biosci 2011;3:711-35.
Uskoković V, Bertassoni LE. Nanotechnology in dental sciences:
Moving towards a finer way of doing dentistry. Materials (Basel)
;3:1674-91.
Niu LN, Zhang W, Pashley DH, Breschi L, Mao J, Chen JH, et al.
Biomimetic remineralization of dentin. Dent Mater 2014;30:77-96.
Thula TT, Svedlund F, Rodriguez DE, Podschun J, Pendi L, Gower LB.
Mimicking the nanostructure of bone: Comparison of polymeric
process-directing agents. Polymers (Basel) 2011;3:10-35.
Zhao J, Liu Y, Sun WB, Zhang H. Amorphous calcium phosphate and
its application in dentistry. Chem Cent J 2011;5:40.
Bacino M, Girn V, Nurrohman H, Saeki K, Marshall SJ, Gower L, et al.
Integrating the PILP-mineralization process into a restorative dental
treatment. Dent Mater 2019;35:53-63.
Published
How to Cite
Issue
Section
