ANTIOXIDANT ACTIVITY TEST OF FRACTIONS FROM STAR FRUIT LEAVES (AVERRHOA CARAMBOLA L.) FROM THREE REGIONS IN WEST JAVA
Keywords:Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, Fractionation, Ferric reducing ability of plasma, Star fruit
Objectives: Previous in vitro research showed that ethyl acetate fractions of star fruit leaves exhibit strong antioxidant activities toward 2,2-diphenyl-
1-picrylhydrazyl (DPPH) radical (IC50 = 90 μg/ml). This study’s aim was to evaluate antioxidant activity of fractions from leaf extracts from star fruit
sourced from three different regions in Indonesia (Depok, Sukabumi, and Subang) and, for the most active fraction (that with the highest antioxidant
activity), determine whether there was any correlation with phenolic and flavonoid content.
Methods: Liquid–liquid partitioning, fractions were evaluated for in vitro antioxidant activity using DPPH radical scavenging and ferric reducing
ability of plasma (FRAP) assay.
Results: The ethyl acetate fraction from the Subang region exhibited the strongest radical scavenging activity with both the DPPH assay (IC50 = 96 μg/ml)
and the FRAP assay (FeEAC value = 1405 μmol/g).
Conclusion: Ethyl acetate fractions from star fruit leaves could be used as natural antioxidants. No correlation was found between antioxidant activity
and phenolic or flavonoid content.
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