FORMULATION, ANTIOXIDANT, AND ANTI-AGING ACTIVITY OF RUBUS FRAXINIFOLIUS FRACTION

SULISTYOWATI; BERNA ELYA; SYAMSU NUR; RADITYA ISWANDANA

doi:10.22159/ijap.2024v16i4.51013

Authors

SULISTYOWATI Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0009-0007-2717-1036
BERNA ELYA Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0000-0003-2904-6515
SYAMSU NUR Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia. Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Health, Almarisah Madani University, Makassar, South Sulawesi, Indonesia https://orcid.org/0000-0001-7730-4414
RADITYA ISWANDANA Laboratory of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok-16424, West Java, Indonesia https://orcid.org/0000-0003-2637-3844

DOI:

https://doi.org/10.22159/ijap.2024v16i4.51013

Keywords:

Antioxidant, Gel, Rubus fraxinifolius leaves, Elastase inhibition

Abstract

Objective: The aim of this research was to evaluate the antioxidant activity and elastase inhibition of the Rubus gel fraction as well as its physical stability for 12 weeks. Rubus fraxinifolius leaves were reported to have strong antioxidant and elastase enzyme inhibitory activity.

Methods: Water fraction from ultrasonic-assisted extraction (UAE) of old Rubus leaves was used and formulated into face gel preparations. Antioxidant activity 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and 2,2'-azino-bisphenol S (2,2'-ABPS) (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), elastase inhibition, and stability test during 12 weeks of storage, both at room temperature (30 ± 2 °C) or accelerated temperature (40 ± 2 °C) were performed.

Results: The anti-aging gel showed better antioxidant activity and elastase inhibition on F1 (3%) at both conditions compared to F2 (4%) and F3 (5%). The physical stability test met the requirements. However, there was a slight decrease in antioxidant activity and elastase inhibition after 12 weeks of storage at 30 ± 2 °C and 40 ± 2 °C (F1, F2, and F3).

Conclusion: The F1 met the standards and was relatively stable at 30 ± 2 °C during 12 weeks of storage.

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