ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES
Keywords:Antioxidant, 1, 1-diphenyl-2-picrylhydrazyl, Isolation, Garcinia daedalanthera Pierre
Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanol
extracts of Garcinia daedalanthera Pierre leaves.
Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum column
chromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined using
the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,
recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and its
antioxidant activity was quantitatively tested.
Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 Î¼g/mL. Identification using an AlCl3 sprayer produced
yellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.
Infrared spectrum analysis revealed the presence of âˆ’OH, Câ€“H alkane, C=C aromatic, C=O, and C-O-C groups.
Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibited
antioxidant activity with an IC50 of 5.82 Î¼g/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) after
being sprayed with AlCl3 reagent.
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