ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES

Authors

  • Anisa Puspitaningrum Department of Phytochemical and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Berna Elya Department of Phytochemical and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Katrin . Department of Phytochemical and Pharmacognosy, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

DOI:

https://doi.org/10.22159/ijap.2018.v10s1.61

Keywords:

Antioxidant, 1, 1-diphenyl-2-picrylhydrazyl, Isolation, Garcinia daedalanthera Pierre

Abstract

Objective: This study aimed to isolate and characterize the compounds responsible for the high antioxidant activities of the ethyl acetate and methanol
extracts of Garcinia daedalanthera Pierre leaves.
Methods: In this study, the ethyl acetate extract was obtained by column chromatography, and the methanol extract was obtained by vacuum column
chromatography. The mobile phase comprised n-hexane, ethyl acetate, and methanol with increased polarity. Antioxidant activity was examined using
the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The fraction with the highest antioxidant activity was purified through column chromatography,
recrystallization, and preparative thin-layer chromatography. This fraction, termed the isolate of B, was identified using DPPH and AlCl3, and its
antioxidant activity was quantitatively tested.
Results: From this research, 21.7 mg of the isolate of B were obtained with an IC50 of 5.82 μg/mL. Identification using an AlCl3 sprayer produced
yellow phosphorescent spots under UV light. UV-Vis spectrum analysis revealed the presence of an aromatic compound and conjugated double bonds.
Infrared spectrum analysis revealed the presence of −OH, C–H alkane, C=C aromatic, C=O, and C-O-C groups.
Conclusion: Based on this research, 21.7 mg of the isolate of B was derived through fractionation of the methanol extract, and this isolate exhibited
antioxidant activity with an IC50 of 5.82 μg/mL. The isolate of B was considered to be a flavonoid, as it was fluorescent under UV light (366 nm) after
being sprayed with AlCl3 reagent.

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References

Okoko T. In vitro antioxidant and free radical scavenging activities of

Garcinia kola seeds. Food Chem Toxicol 2009;47:2620-3.

Hariyanto S. The diversity, distribution and potential of Garcinia

species in Indoensia. Berkala Penelitian Hayati 2007;12:129-35.

Sari R, Hanan A. Garcinia (Clusiaceae) at Bogor Botanical Gardens

Physiognomy, Diversity, and Potential. Bogor: Hari Cipta Puspa and

Satwa Kebun Raya Bogor Proceeding; 2000.

Elya B. Isolation and characterization of chemical compounds from

n-hexane extract of Garcinia rigida bark. J Makara Sains 2003;7:45-51.

Halliwell B. Handbook of Antioxidants. 2nd ed. Singapura: Taylor and

Francis Group;2002.

Fu M, Feng H, Chen Y, Wang D, Yang G. Antioxidant activity of

Garcinia xanthochymus leaf, root, and fruit extracts in vitro. Chin J Nat

Med 2012;10:129-34.

Kothari S, Thompson A, Agarwal A, Du Plessis Stefan S. Free radicals:

Their beneficial and detrimental effects on sperm function. Indian J Exp

Biol 2010;48:425-35.

Erawati. Antioxidant Activity Test of Garcinia daedalanthera Pierre

Extract with DPPH Method and Identification of Chemical Compounds

from the Most Active. Thesis. Depok: Faculty of Pharmacy, Universitas

Indonesia; 2012.

Mercy LS, Gnanamani A, Ilavarasan R. Physico-chemical,

phytochemical and high performance thin layer chromatography

analysis of the whole plant of Orthosiphon thymiflorus (roth.) sleesen.

Asian J Pharm Clin Res 2015;8:181-4.

Rohini RM, Amit KD. Determination of lupeol, B-sitosterol and

quercetin from ethyl acetate extract of Rhizophora Mucronata bark by

HPTLC technique. Asian J Pharm Clin Res 2011;4:103-5.

Ambarwati NS, Malik A, Deborah EA, Cakra HA, Hanif M, Elya B,

et al. The antibacterial activity of fractions of Ethyl acetate Garcinia

latissima miq. Stem bark extract against Bacillus subtilis and

Pseudomonas aeruginosa. Asian J Pharm Clin Res 2017;10:69-72.

Purwaningsih Y, Ersam T. The compound of xanthones as an antioxidant of

wood sticks Garcinia tetandra Pierre. Akta Kimia Indones 2007;2:103-8.

Margono SA, Zandrato RN. Synthesis of diasetil gamavuton-0 by

using acetyl chloride as acylating agent. Majalah Farmasi Indones

;17:25-31.

Supratman U. Elucidation of Structure of Organic Compounds,

Spectroscopic Method to Determine the Structure of Organic

Compounds. Jakarta: Widya Padjadjaran; 2010. p. 66-7.

Published

20-12-2018

How to Cite

Puspitaningrum, A., Elya, B., & ., K. (2018). ISOLATION AND CHARACTERIZATION OF ANTIOXIDANT COMPOUNDS FROM ETHYL ACETATE AND METHANOL EXTRACTS OF GARCINIA DAEDALANTHERA PIERRE LEAVES. International Journal of Applied Pharmaceutics, 10(1), 276–278. https://doi.org/10.22159/ijap.2018.v10s1.61

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