SYNTHESIS OF BLACK TURMERIC EXTRACT NANOPARTICLES (CURCUMA CAESIA) AND ITS CYTOTOXIC ACTIVITY ON T47D CELLS

Authors

  • MUHAMMAD DA’I Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia https://orcid.org/0000-0003-3083-7875
  • NUR AZIZAH Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia
  • ANDREA Y RAHMANA Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia
  • SETYO NURWAINI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia https://orcid.org/0000-0002-3166-7348
  • ERINDYAH R WIKANTYASNING Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024.v16s6.SE2029

Keywords:

Curcuma caesia, Nanoparticles, Cancer, Cytotoxic, T47D

Abstract

Objective: This study aimed to formulate black turmeric into nanoparticle preparations with various concentrations of chitosan and determine its cytotoxic effect on T47D breast cancer cells.

Methods: Extraction was carried out by the maceration method. Black turmeric condensed extract was formulated into nanoparticles using the ionic gelation method, which was a method that relies on the cross-linking agent sodium tripolyphosphate (Na-TPP). The cytotoxic activity of black turmeric extract was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method.

Results: The results showed that black turmeric extract nanoparticles have a size range of 266-558 nm and were positively charged with zeta potential values ​​ranging from 3.3 to 9.7 mV. The encapsulation efficiency of black turmeric extract nanoparticles was 63.42%. The results of the cytotoxic test showed that both black turmeric extract and black turmeric extract nanoparticles showed moderate cytotoxic activity, with the IC50 values ​​of the two preparations were 78.60 µg/ml and 162.95 µg/ml, respectively.

Conclusion: The results obtained in this research indicate a promising potential of nanoparticles of black turmeric extract as a cytotoxic agent for the treatment of breast cancer.

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Published

18-11-2024

How to Cite

DA’I, M., AZIZAH, N., RAHMANA, A. Y., NURWAINI, S., & WIKANTYASNING, E. R. (2024). SYNTHESIS OF BLACK TURMERIC EXTRACT NANOPARTICLES (CURCUMA CAESIA) AND ITS CYTOTOXIC ACTIVITY ON T47D CELLS. International Journal of Applied Pharmaceutics, 16(06), 28–32. https://doi.org/10.22159/ijap.2024.v16s6.SE2029

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