SYNTHESIS OF BLACK TURMERIC EXTRACT NANOPARTICLES (Curcuma caesia) AND ITS CYTOTOXIC ACTIVITY ON T47D CELLS

MUHAMMAD DA’I; NUR AZIZAH; ANDREA Y RAHMANA; SETYO NURWAINI; ERINDYAH R WIKANTYASNING

doi:10.22159/ijap.2024.v16s6.52781

Authors

MUHAMMAD DA’I Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia https://orcid.org/0000-0003-3083-7875
NUR AZIZAH Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia
ANDREA Y RAHMANA Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia
SETYO NURWAINI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia https://orcid.org/0000-0002-3166-7348
ERINDYAH R WIKANTYASNING Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Jl. A. Yani 157 Pabelan, Kartasura, Sukoharjo-57169, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024.v16s6.52781

Keywords:

Curcuma caesia, Nanoparticles, Cancer, Cytotoxic, T47D

Abstract

Objective: This study aimed to formulate black turmeric into nanoparticle preparations with various concentrations of chitosan and determine its cytotoxic effect on T47D breast cancer cells.

Methods: Extraction was carried out by the maceration method. Black turmeric condensed extract was formulated into nanoparticles using the ionic gelation method, which was a method that relies on the cross-linking agent sodium tripolyphosphate (Na-TPP). The cytotoxic activity of black turmeric extract was tested using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method.

Results: The results showed that black turmeric extract nanoparticles have a size range of 266-558 nm and were positively charged with zeta potential values ranging from 3.3 to 9.7 mV. The encapsulation efficiency of black turmeric extract nanoparticles was 63.42%. The results of the cytotoxic test showed that both black turmeric extract and black turmeric extract nanoparticles showed moderate cytotoxic activity with the IC₅₀ values of the two preparations were 78.60 µg/ml and 162.95 µg/ml, respectively.

Conclusion: The results obtained in this research indicate a promising potential of nanoparticles of black turmeric extract as a cytotoxic agent for the treatment of breast cancer

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