ENHANCING CELL METABOLIC ACTIVITY USING MICROPARTICLES CONTAINING BEETROOT (BETA VULGARIS, LINN) EXTRACT

ANITA SUKMAWATI; SETYO NURWAINI; JIHAN NAUFA AZIMAH; ANISA JEVI ROMANDANI SAPUTRI

doi:10.22159/ijap.2024v16s5.52465

Authors

ANITA SUKMAWATI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia https://orcid.org/0000-0002-9438-955X
SETYO NURWAINI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia https://orcid.org/0000-0002-3166-7348
JIHAN NAUFA AZIMAH Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia
ANISA JEVI ROMANDANI SAPUTRI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia

DOI:

https://doi.org/10.22159/ijap.2024v16s5.52465

Keywords:

Antioxidant, Beta vulgaris, Linn, Chitosan, Ethyl cellulose, Microparticle, metabolic activity, Proliferation

Abstract

Objective: The aim of this study was to assess the efficacy of delivering beetroot (Beta vulgaris, Linn) juice extract, which contains antioxidants, using Ethyl Cellulose (EC) and chitosan microparticles on cell metabolic activity. Methods: The beetroot extract microparticles were produced by using EC and chitosan as a matrix. Different concentrations of the matrix were used in the primary solution during microparticles preparation. The physical characterisation was conducted including Scanning Electron Microscope (SEM) and zeta potential. The entrapment of the active substance was measured using the Encapsulation Efficiency (EE) and Drug Loading (DL). The Vero cell was treated with EC and chitosan microparticles for 28 days and the cell metabolic activity was measured using resazurin assay. Results: The findings indicated that the entrapment of beetroot extract in microparticles was affected by the concentration of EC and chitosan. The delivery of an antioxidant substance from beetroot extract resulted in an increase in cell metabolic activity indicated by cells proliferation from day 7 to day 21, in comparison to the control group.

Conclusion: The antioxidant as an active compound from beetroot juice extract were successfully delivered to the cell via the EC and chitosan microparticle indicated by impact on metabolic activity. The metabolic activity of the cell is influenced by the quantity of active substance contained within the microparticle and the type of polymer used as the microparticle matrix. The EC microparticle demonstrated a greater capacity to stimulate cell metabolic activity in comparison to chitosan microparticles

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