INVESTIGATING BETANIN STABILITY, RELEASE PROFILE AND ANTIOXIDANT ACTIVITY OF ETHYL CELLULOSE MICROPARTICLE CONTAINING BEETROOT (BETA VULGARIS, LINN) EXTRACT

ANITA SUKMAWATI; SETYO NURWAINI; UMI BUDI RAHAYU; APRILIANA P. C. WIDAWAN; ANITA SAFITRI; NOVIA W. N. ASTRIA

doi:10.22159/ijap.2021v13i6.42848

Authors

ANITA SUKMAWATI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia
SETYO NURWAINI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia
UMI BUDI RAHAYU Faculty of Health Sciences, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia
APRILIANA P. C. WIDAWAN Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia
ANITA SAFITRI Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia
NOVIA W. N. ASTRIA Faculty of Pharmacy, Universitas Muhammadiyah Surakarta, Sukoharjo, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021v13i6.42848

Keywords:

Antioxidant, Beta vulgaris Linn,, Ethylcellulose, Microparticle, Release kinetic stability

Abstract

Objective: The objective of this research is to evaluate the ability of ethyl cellulose (EC) microparticle to protect the beetroot (Beta vulgaris, Linn) active substance. In addition, this research also investigates the effect of polymer concentration during microparticle preparation toward physical characteristics of microparticle, release profile of betanin as well as antioxidant activity of microparticle.
Methods: The microparticle was produced using the emulsification method using various concentrations of EC in the organic phase and beetroot extract as the active substances. The physical characterization was carried out including the imaging of microparticle using scanning electron microscope (SEM), zeta potential and encapsulation efficiency (EE). The stability test for an active substance in microparticle was carried out at temperature 40 °C for 28 d. The release profile was evaluated using the dissolution method and the antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH).
Results: The result showed that the EC concentration strongly influenced the physical characteristics and EE of beetroot extract in microparticle. The microparticles also had good protection for betanin during storage. The release of active substance from microparticle following Higuchi kinetic. The highest antioxidant activity was found in the microparticle using EC 20%.
Conclusion: The EC microparticle is the potential to protect the degradation of antioxidant substance from natural product. However, the physical properties, EE, the ability to prevent degradation of active substance, release rate and antioxidant activity, are strongly influenced by the EC polymer concentration during microparticle preparation.

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