FORMULATIONS AND CHARACTERIZATIONS OF TELANG FLOWER EXTRACT (CLITORIA TERNATEA L.) GEL USING HYDROXY PROPYL METHYL CELLULOSE (HPMC) AS GELLING AGENT AND ITS ANTIOXIDANT ACTIVITY
DOI:
https://doi.org/10.22159/ijap.2024.v16s6.52783Keywords:
Telang flowers extract, HPMC, AntioxidantAbstract
Objective: Telang flowers (Clitoria ternatea L.) are plants that contain a natural source of antioxidants that can be formulated in gel preparation with a variation of concentration of gelling agent Hydroxy Propyl Methyl Cellulose (HPMC). The purpose of this research was to know the difference in the levels of HPMC as a gelling agent against the physical properties and antioxidant activity of preparation of telang flower extract gel.
Methods: The extraction of telang flowers is done by a maceration method with a 96% ethanol solvent. The gel was formulated with HPMC concentration variations of 2%, 2.5% and 3% respectively. The physical properties of the gel that were determined were organoleptic, pH, stickiness, spreadability, viscosity, and homogeneity. Antioxidant activity was determined using DPPH (2,2-diphenyl-1-picrylhydrazyl). Data was analyzed statistically using a one-way ANOVA test with a 95% confidence level.
Results: The results of the research showed that the best formula was formula II with a concentration of HPMC of 2.5%. The gel of extract telang flower has characteristics with a typical scent of the telang flower, the blue-purple color, homogeneous, pH was 6.393±0.01, stickiness was 1.203±0.7 s, spreadability was 4.17±0.1 cm, and viscosity was 3000±100 cP. The antioxidant activity (IC50) the gel was 101.42 ppm belongs to the category of moderate antioxidants.
Conclusion: Gel extract of telang flowers with concentration gelling agent HPMC concentration of 2.5% had the best physical properties.
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References
Rasyadi Y, Rahim F, Devita S, Merwanta S, Hanifa D. Formulation and stability test handbody lotion ethanol soursop leaves extract (Ammona muricata Linn.). J Ilmiah Farm. 2022;11(1):15–23. http://dx.doi.org/10.31591/pjif.v11i1.2958
Sari A N. Alternative antioxidants to eliminate the harm of free radicals on the skin. Elkawnie: Journal of Islamic Science Technology. 2015;1(1):63–8. Available from: www.jurnal.ar-raniry.com/index.php/elkawnie
Sapiun Z, Wicita PS, Buloto AS, Kamba V, Imran AK, Buana WAA, et al. Preparation and characterization of butterfly pea (Clitoria ternatea L.) nanoemulgel. Macedonian Journal of Medical Sciences. 2022;10(A):585–589.
Oguis GK, Gilding EK, Jackson MA, Craik DJ. Butterfly pea (Clitoria ternatea), A cyclotide-bearing plant with applications in agriculture and medicine. Frontiers in Plant Science. 2019;10:1–23.
Jeyaraj EJ, Lim YY, Choo WS. Extraction methods of butterfly pea (Clitoria Ternatea) flower and biological activities of its phytochemicals. J Food Sci Technol. 2021;58(6):2054–2067. Available from: https://doi.org/10.1007/s13197-020-04745-3.
Setyawan R, Masrijal CDP, Hermansyah O, Rahmawati S, Intan R, Sari P, et al. Formulation evaluation and physical stability test preparation gel antioxidant extract rope princess (Cassytha filiformis L). Bencoolen Journal of Pharmacy. 2023;3(1):27–33. Available from: https://ejournal.unib.ac.id/index.php/bjp/index
Wiyono AS, Lestari TP, Wardani VS. The influence of HPMC as a gelling agent on the optimization of the formula gel extract crude bromelin pineapple (Ananas comossus L. Merr) skin. J Sint Penelit Sains, Terap dan Anal. 2020;1(2):52–9.
Rowe RC, Sheskey PJ, Quinn ME. Handbook of pharmaceutical excipients sixth edition. Washington dan London: American Pharmaceutical Association and the Pharmaceutical Society of Great Britain. 2009.
Widhiardani FAF, Setiyadi G. Optimization of glycerol as a humectant and HPMC as a geling agent in the antioxidant gel formula of carrot extract (Daucus carota L.). Usadha Journal of Pharmacy. 2023;2(3):278–290. https://doi.org/10.23917/ujp.v2i3.86
Valentina FE, and Saryanti D. Formulation of an antibacterial gel extract of pandanus leaf (Pandanus amaryllifolius Roxb.) using hydroxy propyl methyl cellulose (HPMC) and activity testing against Staphylococcus aureus. Pharmacon: Jurnal Farmasi Indonesia. 2023;20(1):1–9. http://dx.doi.org/10.23917/pharmacon.v20i1.18328
Yusuf AL, Nurawaliah E, Harun N. The effectiveness test of gel of ethanol extract of Moringa oleifera leaves (Moringa oleifera L.) as antifungal Malassezia furfur. Kartika Jurnal Ilmiah Farmasi. 2017;5(2):62–67.
Seru E R, Edy H J, Siampa J P. Formulation of HPMC as a geling agent gel of ethanol extract of leilem leaves (Clerodendrum Minahassae Teisjm Dan Binn.) and antioxidant effectiveness test. Pharmacon. 2021;10(3):1033–1039.
Farhan M, Putriana R A, Humaidi F. Formulation and physical quality test preparation of gel extract of telang flower (Clitoria ternatea L.) as a hand antiseptic. Jurnal Farmasi dan Herbal. 2023;5(2):1–12. http://ejournal.delihusada.ac.id/index.php/JPFH
Cahyaningsih E, Putu E S K, Santoso P. Phytochemical screening and antioxidant activity of telang flower extract (Clitoria ternatea L.) using UV-Vis spectrophotometry. Jurnal Ilmiah Medicamento. 2019;5(1):51–57.
Dewi I S, Septawati T, Rachma FA. Phytochemical screening of tamarillo peel and seeds ethanol extracts ( Solanum betaceum Cav.). Prosiding Seminar Nasional UNIMUS. 2021;4:1210–1218.
Setiawati R, Sukmawati A. Physical characterization and antioxidant activity of peel off gel face mask containing Hylocerus polyrhizus fruit juice. Pharmacon: Jurnal Farmasi Indonesia. 2018;15(2):65–74.
Ardana M, Aeyni V, Ibrahim A. Formulation and optimization of HPMC (Hydroxy Methyl Cellulose) gel bases with various concentration variations. J Trop Pharm Chem. 2015;3(2):101–108.
Nurlely, Rahmah A, Ratnapuri PH, Srikartika VM, Anwar K. Physical characteristics test preparation of gel extract Chromolaena odorata leaves with variations of carbopol and HPMC. J Pharmascience. 2021;8(2):79–89.
Setiyadi G, Qonitah A. Optimization of gel peel-off mask ethanolic extract of betle leaf (Piper betle L.) with carbomer and polivinil alcohol combination. Pharmacon: Jurnal Farmasi Indonesia. 2020;17(2):174–183.
http://dx.doi.org/10.23917/pharmacon.v17i2.11976
Imanto T, Prasetiawan R, Wikantyasning ER. Formulation and characterization of nanoemulgel containing (Aloe vera L.) powder. Pharmacon: Jurnal Farmasi Indonesia. 2019;16(1):28–37. http://dx.doi.org/10.23917/pharmacon.v16i1.8114
Dhiafanti YA, Setiyadi G. Optimization of HPMC as a gelling agent and glycerin as a humectant in gel mask preparation of chayote (Sechium edule (Jacq.) Swartz). Usadha Journal of Pharmacy. 2023;2(1):1–15. https://doi.org/10.23917/ujp.v2i1.93
Rauf J, Isa I, Thomas NA. Formulation of emulgel preparation of kelor leaf extract (Moringa oleifera Lam) and antioxidant activity test using DPPH method. Indonesia Journal Pharmaceutical Education. 2021;1(1):10–19.
Ramdhini RN. Standardization of the quality of simplisia and ethanol extracts of telang flowers (Clitoria ternatea L.). J Kesehat J Ilm Multi Sci. 2023;13(1):32–8.
Indriaty S, Rizikiyan Y, Firmansyah D. Formulation and stability testing of anti-aging gel from a combination of ethanol extract of red dragon (Hylocereus polyrhizus) skin and bacon mucus (Achatina fulica) with gelling agent variations of carbomer 940 1%, 1.25%, 1.5%, and 1.75%. J Pharmacopolium. 2019;2(2):104–111.
Afifah H and Nurwaini S. Testing anti-fungal activity of 934 carbopol-based Aloe vera gel powder against Candida albicans dan Trichophyton mentagrophytes. Pharmacon J Farm Indones. 2018;15(2):42–51. http://dx.doi.org/10.23917/pharmacon.v15i2.7658
Nurwaini S, Hafidzatun Nasihah R. Formulation and testing of antibacterial activity preparation hand gel extract of rose leaf seed (Psidium guajava L.). 7th Univ Res Colloqium 2018 STIKES PKU Muhammadiyah Surakarta. 2018;24–30.
Dwiastuti R, Ardiyati S.E.A. Formulation of nanoparticles gel of binahong leaf extract (Anredera cordifolia (Ten.) Steenis). Pharmacy Medical Journal. 2020;3(2):40–46.
Senja RY, Amelia R. Formulation and test stability gel ethanol extract of kemangi leaves (Ocimum sanctum L.) with gelling agent carbopol 940 concentration 0.5% and 0.75% and HPMC. Medimuh. 2018;1(1):45–54.
Thomas NA, Tungadi R, Hiola F, S. Latif M. The effect of the 940 carbopol concentration as a gelling agent on the physical stability of the Aloe vera gel preparation. Indonesia J Pharm Educ. 2023;3(2):316–324.
Indrawati A, Baharuddin S, Kahar H. Antioxidant activity test of purple plant stem (Graptophylum pictum L.) extract in Takalar district using DPPH visible spectrophotometry. Lumbung Farm J Ilmu Kefarmasian. 2022;3(1):69–77.
Jayanti M, Ulfa AM, Saputra Yasir A. The formulation and physical evaluation tests of ethanol in telang flower (Clitoria ternatea L.) extract losio form as antioxidant. Biomed J Indones. 2021;7(3):448–495. Available from: https://doi.org/10.32539/BJI.v7i3.543
Kristian R, Raharjo S, Sulastrianah. Antioxidant activity test of freshwater microalgae. Medula. 2015;3(1):201–7.
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