ANTIOXIDANT ACTIVITIES AND PHYTOCHEMICAL SCREENING OF ETHANOL EXTRACT FROM SURIAN LEAVES (TOONA SINENSIS)

UCE LESTARI; MUHAIMIN MUHAIMIN; ANIS YOHANA CHAERUNISAA; WAWAN SUJARWO

doi:10.22159/ijap.2023.v15s2.07

Authors

UCE LESTARI Doctoral Study Program, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang-45363, West Java, Indonesia. Department of Pharmacy, Faculty of Medicine and Health Sciences, University of Jambi, Jambi, Indonesia
MUHAIMIN MUHAIMIN Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jawa Barat. Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang-45363, West Java, Indonesia
ANIS YOHANA CHAERUNISAA Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang-45363, West Java, Indonesia
WAWAN SUJARWO Ethnobotany Research Group, Research Center for Ecology and Ethnobiology, National Research and Innovation Agency (BRIN), Jakarta

DOI:

https://doi.org/10.22159/ijap.2023.v15s2.07

Keywords:

Antioxidants, Surian leaves, IC50

Abstract

Objective: The objective of the present study is to determine antioxidant activities and phytochemical screening of ethanol extract from surian leaves (Toona sinensis).

Methods: This study evaluated the antioxidant activity and conducted phytochemical screening of ethanol extracts obtained from surian leaves. Phytochemical screening was performed using qualitative Thin-Layer Chromatography (TLC), and antioxidant activity was assessed using the DPPH method.

Results: The results revealed the presence of polyphenolic compounds (153.10±0.310 mg/g), tannins, flavonoids (33.19±0.890 mg/g), monoterpenoids, quinones, and saponins with Rf values of 0.607±0.019 (flavonoids) and 0.958±0.019 (terpenoids) 0.513±0.038 (steroids) 0.418±0.019 (phenolics). The antioxidant activity test of the ethanol extract from Surian leaves yielded an IC₅₀value of 12.351±0.092 ppm, which closely matches the IC₅₀ value of the reference vitamin C (7.805±0.686 ppm).

Conclusion: In conclusion, based on research methods, the ethanol extract of surian leaves contains flavonoid and phenolic compounds which show strong antioxidant activity.

Downloads

Download data is not yet available.

References

Vaiserman A, Koliada A, Zayachkivska A, Lushchak O. Nanodelivery of natural antioxidants: an anti-aging perspective. Front Bioeng Biotechnol. 2019;7:447. doi: 10.3389/fbioe.2019.00447, PMID 31998711.

Moline MP, Aguirre ME, Dominguez E, Moran Giardini P, Fernandez NJ, Damiani N. Short communication: ascorbyl/ascorbate ratio as a marker of oxidative stress in larvae (Apis mellifera) exposed to paenibacillus larvae. Comp Biochem Physiol B Biochem Mol Biol. 2023;269:110896. doi: 10.1016/j.cbpb.2023.110896, PMID 37640200.

Prasad KN. A micronutrient mixture with collagen peptides, probiotics, cannabidiol, and diet may reduce aging and development and progression of age-related Alzheimer’s disease and improve its treatment. Mech Ageing Dev. 2023;210:111757. doi: 10.1016/j.mad.2022.111757, PMID 36460123.

Ferdous R, Islam MB, Al-amin MY, Dey AK, Mondal MOA, Islam MN. Anticholinesterase and antioxidant activity of drynaria quercifolia and its ameliorative effect in scopolamine-induced memory impairment in mice. J Ethnopharmacol. 2023;319(1):117095. doi: 10.1016/j.jep.2023.117095, PMID 37634747.

Bordim J, Marques C, Calegari MA, Oldoni TLC, Mitterer Daltoe ML. Potential effect of naturally colored antioxidants from Moringa oleifera, propolis, and grape pomace–evaluation of color and shelf life of chicken pate. Food Chemistry Advances. 2023;3. doi: 10.1016/j.focha.2023.100409.

ALaqeel NK. Antioxidants from different citrus peels provide protection against cancer. Braz J Biol. 2023;84:e271619. doi: 10.1590/1519-6984.271619, PMID 37436265.

Joshi K, Kumar P, Kataria R. Microbial carotenoid production and their potential applications as antioxidants: a current update. Pro Biochem. 2023;128:190-205.

Min W, Jianhang C, Zhengfeng F, Shanshan L, Zhiqing H, Zizhong T. Composition and antiaging activities of polyphenol extract from Phyllanthus emblica L. Fruit. Nutrients. 2022;14:857-75.

Nurhamidah N, Nurdin H, Manjang Y, Dharma A. Identification of phytochemical profiles and antioxidant activity tests of Surian leaf diethyl ether extract (Toona sinensis (Juss.) M. Roem.) using the DPPH method. J Pendidik Dan Ilmu Kim. 2019;3(1):65-9.

Wang Y, Gu D, Liu C, Tang S, Wang S, Wang Y. Enrichment, analysis, identification and mechanism of antioxidant components in Toona sinensis. Chin J Anal Chem. 2023;51(9):1-10. doi: 10.1016/j.cjac.2022.100198.

Ying C, Hong G, Xiaoxiao L, Jinyi Z, Yijin J, Feng. Terpenoids from the seeds of Toona sinensis and their ability to attenuate high glucose-induced oxidative stress and inflammation in rat glomerular mesangial cells. Molecules. 2022;27(5784):1-12.

Taslim T, Pratama RH. Analysis of antioxidant power of secondary metabolite compounds of ethanol extract of Surian leaves (Toona sinensis (Juss.) M. Roem.). J Akad Farm Pray. 2020;5(2):9-28.

Mrabti HN, Hachlafi NE, Al-Mijalli SH, Jeddi M, Elbouzidi A, Abdallah EM. Phytochemical profile, assessment of antimicrobial and antioxidant properties of essential oils of Artemisia herba-alba Asso., and Artemisia Dracunculus L.: experimental and computational approaches. J Mol Struct. 2023;1294:129415. doi: 10.1016/j.molstruc.2023.136479.

Akili S, Ben HD, Bitar Y, Chehna M. The new use of high-performance liquid chromatography technology to follow the stages of synthesis of sulfonamides and the calculation of their yield directly. Lett Appl Nanobiosci. 2023;12(430):1-25.

Zhao X, Hu X, Xie Q, Qi S, Xiang Z, Sun X. Ameliorative effect of scopolamine-induced cognitive dysfunction by fufangmuniziqi formula: the roles of alkaloids, saponins, and flavonoids. J Ethnopharmacol. 2024;318(A):116792. doi: 10.1016/j.jep.2023.116792, PMID 37356745.

Lima A, Dias E, Reis I, Carneiro K, Pinheiro A, Nascimento A. Ferulic acid as major antioxidant phenolic compound of the Tetragonisca angustula honey collected in vera cruz-itaparica island, bahia. Brazil Braz J Biol. 2023:842024.

Ministry of Health of the Republic of Indonesia. General Standard parameters of medicinal plant extracts. Jakarta. Director General of POM; 2000.

Ministry of Health of the Republic of Indonesia. Indonesian materia Medika volume VI, directorate general of drug and food control. Jakarta; 1995.

Moulick SP, Jahan F, Mamun MZUA, Hossain MIS, Waliullah M, Sathee RA. Analysis of indigenous spices widely consumed in Bangladesh: an assessment to explore its proximate contents, minerals, phytochemical compositions, and antioxidant activities. Journal of Agriculture and Food Research. 2023;14:100720. doi: 10.1016/j.jafr.2023.100720.

Landes FC, Calcio M, Sobolewski JM, Wallens Logan K, Ledeczi A, Kiro Y. Widespread Pb contamination in urban backyard soils for >100 years identified in soil cores constrained by 210Pb and 137Cs. Sci Total Environ. 2023;899. doi: 10.1016/j.scitotenv.2023.165407.

Su S, Wang L, Ni J, Geng Y, Xu X. Diversity of red, green and black cultivars of Chinese toon [Toona sinensis (A. Juss.) roem]: anthocyanins, flavonols and antioxidant activity. Food Measure. 2020;14(6):3206-15. doi: 10.1007/s11694-020-00560-8.

Demasi S, Caser M, Scariot V. Hot and cold drying of edible flowers affect metabolite patterns of extracts and decoctions. Folia Horticul. 2023;35(1):193-207. doi: 10.2478/fhort-2023-0015.

Ouyang J, Fan K, Li Q, Wang F, Li W, Su X. Mechanism of feed moisture levels in extrusion treatment to improve the instant properties of Chinese yam (Dioscorea opposita Thunb.) flour. Food Chem. 2024;431:137056. doi: 10.1016/j.foodchem.2023.137056, PMID 37573749.

Abbo HS, Hung Lai C, Titinchi SJJ. Substituent and solvent effects on UV‐visible absorption spectra of chalcones derivatives: experimental and computational studies. Spectrochim Acta A Mol Biomol Spectrosc. 2023;303:123180. doi: 10.1016/j.saa.2023.123180, PMID 37517269.

Mughal TA, Ali S, Hassan A, Kazmi SAR, Saleem MZ, Shakir HA. Phytochemical screening, antimicrobial activity, in vitro and in vivo antioxidant activity of berberis lycium royle root bark extract. Braz J Biol. 2022;84:e249742. doi: 10.1590/1519-6984.249742, PMID 35475988.

Chang CC, Yang MH, Wen HM, Chernn JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal. 2002:178-82.

Moulick SP, Jahan F, Mamun MZUA, Hossain MIS, Waliullah M, Sathee RA. Analysis of indigenous spices widely consumed in Bangladesh: an assessment to explore its proximate contents, minerals, phytochemical compositions, and antioxidant activities. Journal of Agriculture and Food Research. 2023;14:100720. doi: 10.1016/j.jafr.2023.100720.

Saifudin A, Rahayu V, Teruna HY. Standardization of natural medicinal ingredients. Yogyakarta, Graha Ilmu; 2011.

Rong D, Liu Z, Huang J, Zhang F, Wu Q, Dai J. Prevalence and characterization of Staphylococcus aureus and Staphylococcus argenteus isolated from rice and flour products in Guangdong, China. Int J Food Microbiol. 2023;406:110348. doi: 10.1016/j.ijfoodmicro.2023.110348, PMID 37573713.

Haider Z, Qamer S, Kanwal S, Manzoor S, Naeem M, Uddin J. Assessment of essential minerals and physico-chemical analysis of floral origins fresh honey produced by Apis mellifera. Braz J Biol. 2022;84:e263534. doi: 10.1590/1519-6984.263534, PMID 35857954.

Luo X, Xiang C, Wu C, Gao W, Ke W, Zeng J. Geochemical fractionation and potential release behavior of heavy metals in lead‒zinc smelting soils. Journal of Environmental Sciences. 2024;139:1-11. doi: 10.1016/j.jes.2023.05.022.

Kartoglu B, Bahcivan A, Erarpat S, Bayraktar A, Bakirdere S. Microwave-assisted synthesis method for cobalt nanoleaves and its usage in sensitive determination of lead in blue butterfly tea extract and tap water samples by flame atomic absorption spectrophotometry. J Food Com. 2023;15(429):136862.

Lestari U, Muhaimin M, Chaerunisaa AY, Sujarwo W. Anti-aging potential of plants of the Anak dalam tribe, Jambi, Indonesia. Pharmaceuticals (Basel). 2023;16(9):103390. doi: 10.3390/ph16091300, PMID 37765107.

Lestari U, Yuliawati Y, Sani F, Yuliana Y, Muhaimin M. Antioxidant activities of scrub body lotion extract of Surian leaves (Toona sinensis) with powder scrub date seeds (Phoenix dactylifera). IJPST. 2023;1(1):60-9. doi: 10.24198/ijpst.v1i1.42828.

Lestari U, Muhaimin M, Yuhana Y. Physical properties of peel-off gel mask ethanol extract of Surian leaves (Toona sinensis) as an antioxidant. IJPST. 2023;1(1):90-9.

Yang L, Zhang F, He W, Zhao B, Zhang T, Wang S. Extraction optimization and constituent analysis of total flavonoid from Hosta plantaginea (Lam.) Aschers flowers and its ameliorative effect on chronic prostatitis via inhibition of multiple inflammatory pathways in rats. J Ethnopharmacol. 2024;318(A):116922. doi: 10.1016/j.jep.2023.116922, PMID 37516390.