CYTOTOXIC ACTIVITY AND PHYTOCHEMICAL SCREENING OF ETANOL EXTRACT OF BAJAKAH TAMPALA (UNCARIA LANOSA VAR. FERREA (BLUME) RIDSDALE) STEM ON BREAST CANCER CELL LINES MCF-7

MASHURI YUSUF; MUHAIMIN MUHAIMIN; RIEZKI AMALIA; YOPPI ISKANDAR

doi:10.22159/ijap.2023.v15s2.08

Authors

MASHURI YUSUF Faculty of Pharmacy, Padjadjaran University, Sumedang, Indonesia, Department of Pharmacy, Kesuma Bangsa Health Polytechnic, Lampung, Indonesia
MUHAIMIN MUHAIMIN Department of Pharmaceutical Biology, Padjadjaran University, Sumedang, Indonesia
RIEZKI AMALIA Departmen of Pharmacology and Clinical Pharmacy, Padjajaran University, Sumedang, Indonesia
YOPPI ISKANDAR Department of Pharmaceutical Biology, Padjadjaran University, Sumedang, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023.v15s2.08

Keywords:

Bajakah tampala, Cytotoxic, Breast cancer, MCF-7 cells

Abstract

Objective: This study aimed to determine the content of phytochemical screening and cytotoxic activity indicated by the IC₅₀ value of the ethanol extract of bajakah tampala stem.

Methods: Phytochemical screening for extract consists of saponins with distilled water, steroids and terpenoids were determined with the reagent glacial acetic acid and sulphuric acid, tannin using reagent 10%, FeCl₃, alkaloids determined with reagents Mayer, flavonoids using reagent HCl, Mg powder, and phenolic with using 2%. FeCl₃. The WST-8 procedures were used to investigate the cytotoxic activity of the MCF-7 breast cancer cell type.

Results: Based on the results showed that the ethanol extract of bajakah tampala stem has secondary metabolite content, namely the presence of saponins, steroids, tannins, alkaloids, flavonoids and phenolics. The results of the cytotoxic test of ethanol extract of bajakah tampala stem have cytotoxic activity with IC₅₀ of 193.2 mg/ml, which is included in the moderately active category.

Conclusion: In this study, the ethanol extract of bajakah tampala stem has secondary metabolite content and cytotoxic activity against MCF-7 breast cancer cells.

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References

Arsul MI, Syamsi N, Putri N, Nur NAA, Mukhriani M, Hamzah N. Total phenolic, flavonoid, and antioxidant capacity of bajakah (Spatholobus littoralis Hassk). CRBB. 2022;4(1):242-5. doi: 10.5614/crbb.2022.4.1/VRJ3X4LF.

Al-Khedhairy AA, Wahab R. Silver nanoparticles: an instantaneous solution for anticancer activity against human liver (HepG2) and breast (MCF-7) cancer cells. Metals. 2022;12(1):1-18. doi: 10.3390/met12010148.

Alotaibi MR, Hassan ZK, Al-Rejaie SS, Alshammari MA, Almutairi MM, Alhoshani AR. Characterization of apoptosis in a breast cancer cell line after IL-10 silencing. Asian Pac J Cancer Prev. 2018;19(3):777-83. doi: 10.22034/APJCP.2018.19.3.777, PMID 29582634.

Ayuchecaria N, Saputera M, Niah R. Penetapan kadar fenolik total ekstrak batang bajakah tampala (Spatholobus littoralis Hassk.) menggunakan UV-visible. JIFI. 202;3(1):132-41.

Bucar F, Wube A, Schmid M. Natural product isolation-how to get from biological material to pure compounds. Nat Prod Rep. 2013;30(4):525-45. doi: 10.1039/c3np20106f, PMID 23396532.

Chamchoy K, Pakotiprapha D, Pumirat P, Leartsakulpanich U, Boonyuen U. Application of WST-8 based colorimetric NAD(P)H detection for quantitative dehydrogenase assay. BMC Bhiohemsitry J 2019;20(1):1-14.

Chang MS. Tamoxifen resistance in breast cancer. Biomol Ther (Seoul). 2012;20(3):256-67. doi: 10.4062/biomolther.2012.20.3.256, PMID 24130921.

Cheng S, Castillo V, Welty M, Alvarado M, Eliaz I, Temm CJ. Breast Defend enhances the effect of tamoxifen in estrogen receptor-positive human breast cancer in vitro and in vivo. BMC Complement Altern Med. 2017;17(1):115. doi: 10.1186/s12906-017-1621-7, PMID 28209156.

Comşa S, Cimpean AM, Raica M. The story of MCF-7 breast cancer cell line: 40 years of experience in research. Anticancer Res. 2015;35(6):3147-54. PMID 26026074.

Cooper GM. The cell: a molecular approach. ASM Press; 2000.

Susanto BNA, Zayani N. The effect of bajakah (Spatholobus littoralis Hassk) stem extract on calculation of leukocyte in mice (Mus musculus). FP. 2021. p. 1100-6. doi: 10.26911/ICPHmedicine.FP.08.2021.03.

Doyle A, Griffiths JB. Cell and tissue culture: laboratory procedures in biotechnology. Vol. 1. Wiley; 1998.

Fan P, Agboke FA, Cunliffe HE, Ramos P, Jordan VC. A molecular model for the mechanism of acquired tamoxifen resistance in breast cancer. Eur J Cancer. 2014;50(16):2866-76. doi: 10.1016/j.ejca.2014.08.011, PMID 25204804.

Diniatik. Penentuan Kadar flavonoid total ekstrak Etanolik daun Kepel (Stelechocarpus Burahol (Bl.) hook F. and Th.) dengan metode spektrofotometri. J Ilmiah Farmasi. 2015;3:1-5.

Agustina S, Wiraningtyas A, Fitokimia S. Tanaman obat di kabupaten bima. JAC. 2016;4(1):71-6.

Fauziah PC, Nurul H, Hadi I. Usia dan paritas tidak berhubungan dengan ekspresi estrogen reseptor (ER) dan progesteron reseptor (PR) pada kanker payudara invasif no special type (NST) di RSUD abdul wahab sjahranie samarinda: age and parity are not related with estrogen receptor (ER) and progesterone receptor (PR) expression in invasive breast cancer of no special type (NST) at abdul wahab sjahranie hospital. Samarinda JSK. 2022;4(2):126-31. doi: 10.25026/jsk.v4i2.868.

Chen YH, Wu CP, Lin HL, Liaw RB, Lai YY, Wu MC. Tetrazolium salt WST-8 as a novel and reliable chromogenic indicator for the assessment of boar semen quality. Animals (Basel). 2020;10(12):2293. doi: 10.3390/ani10122293, PMID 33291566.

Nissa CK, Oehadian A, Martakusumah AH, Dewi YA. Perbandingan akurasi berbagai formula untuk mengestimasi laju filtrasi glomerulus pada penderita karsinoma nasofaring stadium lanjut sebelum mendapat kemoterapi cisplatin. MKB. 2015;47(1):42-8. doi: 10.15395/mkb.v47n1.396.

Setiawan SI, Safitri EI, Hidayati DN, Muna LN. Aktivitas sitotoksik dan induksi apoptosis dari ekstrak etanol kulit apel hijau (Pyrus malus L.) terhadap Sel MCF-7. JPS. 2021;8(2):69-78. doi: 10.20527/jps.v8i2.10729.

Weerapreeyakul N, Nonpunya A, Barusrux S, Thitimetharoch T, Sripanidkulchai B. Evaluation of the anticancer potential of six herbs against a hepatoma cell line. Chin Med. 2012;7(1):15. doi: 10.1186/1749-8546-7-15, PMID 22682026.

Jacobo Herrera N, Perez Plasencia C, Castro Torres VA, Martinez Vazquez M, Gonzalez Esquinca AR, Zentella Dehesa A. Selective acetogenins and their potential as anticancer agents. Front Pharmacol. 2019;10:783. doi: 10.3389/fphar.2019.00783, PMID 31379567.

Rezk YA, Yang K, Bai S, Mclean K, Johnston C, Reynolds RK. Disulfiram’s antineoplastic effects on ovarian cancer. JCT. 2015;06(14):1196-205. doi: 10.4236/jct.2015.614130.