INHIBITION OF LIPOXYGENASE ACTIVITY FROM AVERRHOA CARAMBOLA L. LEAF EXTRACTS

Authors

  • RANA KURNIA RAHMA Departement of Pharmacognosy and phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • BERNA ELYA Departement of Pharmacognosy and phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia
  • ROSITA HANDAYANI Departement of Pharmacognosy and phytochemistry, Faculty of Pharmacy, Universitas Indonesia, Depok, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2020.v12s1.FF024

Keywords:

Inflammation, Averrhoa carambola L, Lipoxygenase, Apigenin

Abstract

Objective: Inflammation is a protective response to tissue injury caused by physical trauma, harmful chemicals, or microbiological substances.
Leukotriene, which originates in the lipoxygenase pathway, is a mediator of inflammation. Averrhoa carambola L. leave contains flavones, including
apigenin, which exhibits potential anti-inflammatory activity through the inhibition of lipoxygenase activity. The present study aimed to examine the
lipoxygenase inhibition activity of A. carambola leaf extracts.
Methods: A. carambola leaf extracts were obtained using multilevel maceration using the solvents n-hexane, ethyl acetate, and ethanol. Subsequently,
total flavonoid contents in the three extracts were determined using the colorimetric method. Apigenin content in the most active extract was
determined using high-performance liquid chromatography.
Results: The extract with the highest lipoxygenase inhibition activity was the ethyl acetate extract with an IC50 value of 10.17±0.83 μg/mL
followed by the n-hexane and ethanol extracts with IC50 values of 40.99±0.51 and 35.61±0.66 μg/mL, respectively. Total flavonoid content from
the n-hexane, ethyl acetate, and ethanol extracts was 3.01, 24.24, and 8.24 mg quercetin equivalent/g extract, respectively. A qualitative test using
thin-layer chromatography showed that all extracts contained apigenin. The most active (ethyl acetate) extract contained 5.39% apigenin with a
high-performance liquid chromatography method.
Conclusion: The ethyl acetate extract of A. carambola leaf is the most active extract to inhibit lipoxygenase activity. Flavonoid content has a strong
correlation to the inhibitory activity of each extract.

Downloads

Download data is not yet available.

References

1. Corwin EJ. Handbook of Pathophysiology. Philadelphia, PA: Lippincot
Williams and Wilkins; 2008. p. 3.
2. Murray RK, Granner DK, Rodwell VW. Harper’s Biochemistry.
Jakarta: EGC Penerbit Buku Kedokteran; 2012. p. 29.
3. Gilman GO. Basic Pharmacological Therapies. Jakarta: Penerbit Buku
Kedokteran, EGC; 2015. p. 10.
4. Thomas S, Patil DA, Patil AG, Chandra N. Pharmacognostic evaluation
and physicochemical analysis of Averrhoa carambola L. fruit. J Herb
Med Toxicol 2008;2:51-4.
5. Dasgupta P, Chakraborty P, Bala NN. Averrhoa carambola: An update
review. Int J Pharm Res Rev 2013;2:58-63.
6. Cabrini DA, Moresco HH, Imazu P, da Silva CD, Pietrovski EF,
Mendes DA, et al. Analysis of the potential topical anti-inflammatory
activity of Averrhoa carambola L. in mice. Evid Based Complement
Alternat Med 2011;2011:908059.
7. Putri N, Elya B, Puspitasari N. Antioxidant activity and lipoxygenase
inhibition test with total flavonoid content from Garcinia kydia
Roxburgh leaves extract. Phcog J 2017;9:280-4.
8. Fransworth NR. Biological and phytochemical screening of plants.
J Pharm Sci 1996;22:226-76.
9. Ministry of Health, Republic of Indonesia. Supplement II Indonesian
Pharmacopoeia Edition I. Jakarta: Director General of Pharmaceutical
Development and Medical Devices, Ministry of Health, Republic of
Indonesia; 2011.
10. Chedea VS, Ioana S, Socaciu C, Nagaya T, Joseph H, Ogola O.
Lipoxygenase-quercetin interaction: A kinetic study through
biochemical and spectroscopy approaches. Biochem Testing
2012:152-78.
11. Deschamps J, Kenyon V, Holman T. Baicalein is a potent in vitro
inhibitor against both reticulocyte 15-human and platelet 12-human
lipoxygenases. Bioorg Med Chem 2006;14:4295-301.

Published

23-03-2020

How to Cite

RAHMA, R. K., ELYA, B., & HANDAYANI, R. (2020). INHIBITION OF LIPOXYGENASE ACTIVITY FROM AVERRHOA CARAMBOLA L. LEAF EXTRACTS. International Journal of Applied Pharmaceutics, 12(1), 116–118. https://doi.org/10.22159/ijap.2020.v12s1.FF024

Issue

Section

Original Article(s)

Most read articles by the same author(s)

<< < 1 2 3 > >>