IMPROVED SOLUBILITY OF CHOLECALCIFEROL AS BOVINE SERUM ALBUMIN (BSA) NANOPARTICLES

YENNI PUSPITA TANJUNG; MELISA INTAN BARLIANA; I. MADE JONI; ANIS YOHANA CHAERUNISAA

doi:10.22159/ijap.2024v16i1.49422

Authors

YENNI PUSPITA TANJUNG Doctoral program, Faculty of Pharmacy, Padjadjaran University, Sumedang-45363, Indonesia. Bumi Siliwangi Academy of Pharmacy, Bandung, West Java-Indonesia https://orcid.org/0000-0002-1503-7856
MELISA INTAN BARLIANA Center of Excellence in Higher Education for Pharmaceutical Care Innovation, Padjadjaran University, Sumedang-45363, Indonesia. Department of Biological Pharmacy, Faculty of Pharmacy, Padjadjaran University, Sumedang-45363, Indonesia https://orcid.org/0000-0003-0015-9604
I. MADE JONI Department of Physics, Faculty of Mathematics and Natural Science, Padjadjaran University, Sumedang-45363, Indonesia. Functional Nano Powder University Center of Excellence (FiNder U CoE), Padjadjaran University, Sumedang-45363, Indonesia https://orcid.org/0000-0001-5949-3418
ANIS YOHANA CHAERUNISAA Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Padjadjaran University, Sumedang-45363, Indonesia https://orcid.org/0000-0002-4985-8206

DOI:

https://doi.org/10.22159/ijap.2024v16i1.49422

Keywords:

BSA, Cholecalciferol, Nanoparticle, Desolvation, Solubility

Abstract

Objective: This study aims to report the optimum formula for BSA nanoparticles cholecalciferol (BSA-NP cholecalciferol), which can increase the solubility of cholecalciferol.
Methods: BSA cholecalciferol nanoparticles was prepared by desolvation method with variations in solvent/non-solvent ratio, BSA concentration, pH of BSA solution, and cholecalciferol concentration. For this purpose, particle size, polydispersity index, and zeta potential were measured. Furthermore, the solubility test of the best BSA-NPs cholecalciferol formula was carried out.
Results: The most optimal BSA nanoparticle cholecalciferol characterization results have a particle size of 166.6±50.3 nm, a zeta potential of-32.1 mV, and a percentage encapsulation efficiency (%EE) for cholecalciferol of 82.9±0.72%. The solubility of BSA-NP cholecalciferol is four times higher than that of pure cholecalciferol.
Conclusion: The optimum formula for BSA-NP cholecalciferol with a solvent/non-solvent ratio of 1/2, a concentration of BSA of 2.5%, a BSA solution pH 6, and a cholecalciferol concentration of 0.1% will increase the solubility of cholecalciferol by four times compared to pure cholecalciferol.

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